Acrylamide is a chemical used in electrophoresis applications to separate proteins and nucleic acids. During electrophoresis, acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues. It is vital for monomer polymerization.
High exposure to Acrylamide has been linked to nerve damage and reproductive toxicity in humans and has been shown to cause several types of cancer in rodents. To avoid exposure to acrylamide powder, Acrylamide solutions are also available.
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|Microbiology Applications||Acrylamide (and bis-acrylamide) can be synthetically modified into new polymers and compounds with antibacterial properties.|
|Electrophoresis Applications||Acrylamide is most fequently used in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Polyacrylamide gels contain linear acrylamide polymers which form small pores allowing separation of proteins and nucleic acids based on molecular weight.
Our Grade II Acrylamide is produced by enzyme conversion, eliminating the residual by-products produced using chemical synthesis. It is then purified with a proprietary purification method which results in Acrylamide with ultra-low turbidity (<0.5NTU, versus ~2.0 NTU for molecular biology grade Acrylamide) and <0.005% insolubles which ensures high resolution quality.
|Cell Culture Applications||Polyacrylamide hydrogels of varying compliances can be used to review the effect of substrata stiffness on cellular function as part of in vitro models. Tissue stiffness is determinatnt of cellular function, and changes in stiffness can be associate with diseases like fibrosis, cancer, and cardiovascular disease. Polyacrylamide can mimic the rane of tissue compliances seen biologically.|
|Heavy Metals||Copper (Cu) ≤ 250 ppb
Iron (Fe) ≤ 1.0 ppm
Lead (Pb) ≤2 ppm
Cretu A, Castagnino P and Assoian R (2010) Studying the effects of matrix stiffness on cellular function using Acrylamide-based hydrogels. J. Vis. Exp. (42) e2089 doi:10.3791/2089
Zhang A (2015) Synthesis and antimicrobial activities of Acrylamide polymers containing quaternary ammonium salts on bacteria and phytopathogenic fungi. React. Func. Polymers 88:39-46