SKU: A228-10ML  / 
    CAS Number: 7177-48-2

    Ampicillin ReadyMade™ Solution

    $55.00

    Ampicillin ReadyMadeTM solution is provided as a sterile-filtered solution of Ampicillin Trihydrate formulated in water at a concentration of 100 mg/ml. It has been filter-sterilized using a 0.2 μm filter.

    Ampicillin Trihydrate, USP is a member of the β-lactam family similar in structure to penicillin.

    We also offer:

    • Ampicillin Trihydrate, USP (A009)
    • Ampicillin/Sulbactam (2:1)(A071)
    • Ampicillin Anhydrous (A043)
    • Ampicillin Sodium (A042)
    • Ampicillin Trihydrate, EP (A020)

     

    Mechanism of Action Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBPs are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
    Spectrum Ampicillin is broad-spectrum, effective against both Gram-positive and Gram-negative bacteria including Gram-negative non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, Ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to Ampicillin is routinely used as a selectable marker to confirm successful cell transformation.
    Microbiology Applications Ampicillin Trihydrate can be used as a selective agent in several types of isolation media:

    Aeromonas Medium Base - Ampicillin Selective Supplement
    Luria-Bertani (LB) Medium

    Eukaryotic Cell Culture Applications Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL.  For additional information, please visit our cell-culture database.
    Molecular Formula C16H19N3O4S·3H2O (Ampicillin Trihydrate)
    References

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    Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem 52:825-869 PMID 6351730

    Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coli using two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011

    Cure E, Kucuk A and Cure MC (2020) Cyclosporine therapy in cytokine storm due to coronavirus disease 2019 (COVID-19). Rheumatol. Int. 40:1177-1179

    Homan KA et al (2016) Bioprinting of 3D convoluted renal proximal tubules on perfusable chips. Sci Rep. 11(6):34845 PMID 27725720

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    Zhang G and Lyons JG (2002) Cyclosporin A improves the selection of cells transfected with the Puromycin acetyltransferase gene. Biotechniques, 33(1):32-36