|Application||During the first day of life, septicemia commonly occurs in calves. The bacteria that can cause septicemia include E. coli, Salmonella spp, Campylobacter spp, Klebiella spp, and Staphylococcus spp. Rational antibiotic therapy is needed to prevent mortality in calves, and reduce the risk of antibiotic resistance. Cefquinome is safer in the target animals compared to other antibiotics since it is time dependent and it has no or minimal post antibiotic effect. Cefquinome has also been used for acute mastitis, dermatitis, and respiratory diseases (Ahmad et al, 2015).|
|Mechanism of Action||Cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to bacterial cell lysis and death. Although resistance to cefquinome is rare, they are active against bacteria carrying the AmpC-type β-lactamase resistance mechanism.|
|Spectrum||Broad-spectrum targeting Gram-positive and Gram-negative bacteria including E. coli, Pasteurella spp, Salmonella spp, and Enterobacteriaceae spp.|
The antibacterial activity is similar to cefpirome and cefotaxime.
Representative MIC values include:
For a complete list of Cefquinome MIC values, click here.
|Eukaryotic Cell Culture Applications||S. aureus is one of the common pathogens to cause subclinical bovine mastitis. Authors analyzed isolates from milk to determine their capacity to produce biofilm (exopolysaccharidic matrix that aids in adherence and colonization, protecting the bacteria from host immune system and antimicrobial action) on bovine primary mammary epithelial cells. They also tested isolates for susceptibility to cefquinome sulfate in the presence of biofilm. They found that in the presence of biofilm no isolates grew, demonstrating its efficacy. After the biofilm was broken by sonication, all isolates were recovered, confirming the protective effect of biofilm (Castilho et al, 2017).|
|Molecular Formula||C23H24N6O5S2 ∙H2SO4|
|Solubility||Slightly soluble in DMSO and methanol. It degrades in aqueous solutions|
|References||Ahmad I et al (2015) Integration of PK/PD for dose optimization of Cefquinome against Staphylococcus aureus causing septicemia in cattle. Front. Microbiol. 6:588 PMID 26136730 Castiho IG et al (2017) Host-pathogen interactions in bovine mammary epithelial cells and HeLa cells by Staphylococcus aureus isolated from subclinical bovine mastitis. J. Dairy Sci. 100(8):6414-6421 Chin N, Gu J, Fang W and Neu HC (1992) In vitro activity of cefquinome, a new cephalosporin, compared with other cephalosporin antibiotics. Diag. Microbiol. Infect. Dis. 15(4):331-337 PMID 1611848|