SKU: A042  / 
    CAS Number: 69-52-3

    Ampicillin Sodium

    $33.00 - $220.50
    • Detailed Description

      CAS Number: 69-52-3

      Molecular Formula: C16H18N3NaO4S

      Molecular Weight: 371.39

      Mechanism of Action: Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.

      Storage Conditions: 2-8°C

      Tariff Code: 2941.10.1000

    • Applications

      Spectrum: Ampicillin Sodium targets Gram-negative non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."

      Microbiology Applications: Ampicillin Sodium is often used to select for cells that have been transformed with a plasmid containing the bla gene which confers resistance to Ampicillin. Ampicillin Sodium is typically used at a concentration of 50-100 µg/mL.

      Media Supplements

      Ampicillin Sodium can be used as a selective agent in several types of isolation media:

      Aeromonas Medium Base - Ampicillin Selective Supplement

      Eukaryotic Cell Culture Applications: Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    • Specifications

      Form: Powder

      Appearance: White or almost white powder, hygroscopic

      Source: Semi-synthetic

      Water Content (Karl Fischer): ≤2.0%

      pH: 8.0-10.0

      Assay: (anhydrous basis): 845-988 µg/mg

      Identification: Passes test

    • Technical Data

      Solubility: Soluble in water (50 mg/ml)

      Impurities: Dimethylanilline: Meets the requirements
      Methylene Chloride: ≤0.2%

    • References

      References:

      Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am. J. Med 103(1):51-59 PMID 9236486

      Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem 52:825-869 PMID 6351730

      Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coli using two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011

    Ampicillin Sodium is a member of the β-lactam family structurally similar to penicillin. The compound inhibits bacterial cell wall synthesis.  Ampicillin resistance is used as a selectable marker to confirm successful cell transformation, as only cells containing plasmid-encoded ESBLs (Extended Spectrum B-lactamases) survive.   Ampicillin Sodium is soluble in water.

    We also offer:

    • Ampicillin Anhydrous (A043)
    • Ampicillin/Sulbactam (2:1) (A071)
    • Ampicillin Trihydrate, USP (A009)
    • Ampicillin Trihydrate, EP (A020)

    CAS Number: 69-52-3

    Molecular Formula: C16H18N3NaO4S

    Molecular Weight: 371.39

    Mechanism of Action: Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.

    Storage Conditions: 2-8°C

    Tariff Code: 2941.10.1000

    Spectrum: Ampicillin Sodium targets Gram-negative non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."

    Microbiology Applications: Ampicillin Sodium is often used to select for cells that have been transformed with a plasmid containing the bla gene which confers resistance to Ampicillin. Ampicillin Sodium is typically used at a concentration of 50-100 µg/mL.

    Media Supplements

    Ampicillin Sodium can be used as a selective agent in several types of isolation media:

    Aeromonas Medium Base - Ampicillin Selective Supplement

    Eukaryotic Cell Culture Applications: Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    Form: Powder

    Appearance: White or almost white powder, hygroscopic

    Source: Semi-synthetic

    Water Content (Karl Fischer): ≤2.0%

    pH: 8.0-10.0

    Assay: (anhydrous basis): 845-988 µg/mg

    Identification: Passes test

    Solubility: Soluble in water (50 mg/ml)

    Impurities: Dimethylanilline: Meets the requirements
    Methylene Chloride: ≤0.2%

    References:

    Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am. J. Med 103(1):51-59 PMID 9236486

    Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem 52:825-869 PMID 6351730

    Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coli using two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011