-
Detailed Description
CAS Number: 69-52-3
Molecular Formula: C16H18N3NaO4S
Molecular Weight: 371.39
Mechanism of Action: Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
Storage Conditions: 2-8°C
Tariff Code: 2941.10.1000
-
Applications
Spectrum: Ampicillin Sodium targets Gram-negative non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."
Microbiology Applications: Ampicillin Sodium is often used to select for cells that have been transformed with a plasmid containing the bla gene which confers resistance to Ampicillin. Ampicillin Sodium is typically used at a concentration of 50-100 µg/mL.
Media Supplements
Ampicillin Sodium can be used as a selective agent in several types of isolation media:
Aeromonas Medium Base - Ampicillin Selective Supplement
Eukaryotic Cell Culture Applications: Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.
-
Specifications
Form: Powder
Appearance: White or almost white powder, hygroscopic
Source: Semi-synthetic
Water Content (Karl Fischer): ≤2.0%
pH: 8.0-10.0
Assay: (anhydrous basis): 845-988 µg/mg
Identification: Passes test
-
Technical Data
Solubility: Soluble in water (50 mg/ml)
Impurities: Dimethylanilline: Meets the requirements
Methylene Chloride: ≤0.2% -
References
References:
Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am. J. Med 103(1):51-59 PMID 9236486
Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem 52:825-869 PMID 6351730
Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coli using two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011
Ampicillin Sodium is a member of the β-lactam family structurally similar to penicillin. The compound inhibits bacterial cell wall synthesis. Ampicillin resistance is used as a selectable marker to confirm successful cell transformation, as only cells containing plasmid-encoded ESBLs (Extended Spectrum B-lactamases) survive. Ampicillin Sodium is soluble in water.
We also offer:
- Ampicillin Anhydrous (A043)
- Ampicillin/Sulbactam (2:1) (A071)
- Ampicillin Trihydrate, USP (A009)
- Ampicillin Trihydrate, EP (A020)
CAS Number: 69-52-3
Molecular Formula: C16H18N3NaO4S
Molecular Weight: 371.39
Mechanism of Action: Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
Storage Conditions: 2-8°C
Tariff Code: 2941.10.1000
Spectrum: Ampicillin Sodium targets Gram-negative non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."
Microbiology Applications: Ampicillin Sodium is often used to select for cells that have been transformed with a plasmid containing the bla gene which confers resistance to Ampicillin. Ampicillin Sodium is typically used at a concentration of 50-100 µg/mL.
Media Supplements
Ampicillin Sodium can be used as a selective agent in several types of isolation media:
Aeromonas Medium Base - Ampicillin Selective Supplement
Eukaryotic Cell Culture Applications: Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.
Form: Powder
Appearance: White or almost white powder, hygroscopic
Source: Semi-synthetic
Water Content (Karl Fischer): ≤2.0%
pH: 8.0-10.0
Assay: (anhydrous basis): 845-988 µg/mg
Identification: Passes test
Solubility: Soluble in water (50 mg/ml)
Impurities: Dimethylanilline: Meets the requirements
Methylene Chloride: ≤0.2%
References:
Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am. J. Med 103(1):51-59 PMID 9236486
Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem 52:825-869 PMID 6351730
Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coli using two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011