SKU: A071  / 
    CAS Number: 117060-71-6

    Ampicillin/Sulbactam (2:1)

    $185.98

    Ampicillin/Sulbactam (2:1) is a combination of a β-lactam similar in structure to penicillin, with a β-lactamase inhibitor used to increase potency of β-lactam antibiotics.

    We also offer:

    • Ampicillin Anhydrous (A043)
    • Ampicillin Sodium (A042)
    • Ampicillin Trihydrate, USP (A009)
    • Ampicillin Trihydrate, EP (A020) 

     It is used for microbiological in vitro susceptibility tests, and studies in pharmacokinetics and pharmacodynamics.

    Mechanism of Action Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
    Spectrum Ampicillin targets Gram-negative non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus and medically important enteric pathogens such as Shigella and Salmonella. Ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."
    Microbiology Applications Ampicillin/Sulbactam is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.

    Media Supplements

    Ampicillin can be used as a selective agent in several types of isolation media:

    Aeromonas Medium Base - Ampicillin Selective Supplement

    Eukaryotic Cell Culture Applications Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information, please visit our cell-culture database.
    Molecular Formula Ampicillin: C16H19N3O4S
    Sulbactam: C8H11NO5S
    Impurities Dichloromethane: ≤0.06%
    Acetone: ≤0.5%
    Ethanol: ≤0.5%
    Ethyl acetate: ≤0.5%
    References

    Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in Gram-negative bacilli. Am. J. Med 103(1):51-59 PMID 9236486

    Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem. 52:825-869 PMID 6351730

    Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coli using two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011

    MIC Diplococcus pneumoniae| 0.01 - 0.04| 1413| Haemophilus influenzae| 0.04 - 0.8| 1413| Neisseria gonorrhoeae (penicillin-resistant)| 4 - 128| 1408| Neisseria gonorrhoeae (penicillin-susceptible)| 0.008 - 0.25| 1408| Stenotrophomonas maltophilia| >32 - ?| 938|