Ampicillin Trihydrate, USP is a member of the β-lactam family similar in structure to penicillin. Ampicillin Trihydrate is slightly soluble in water (10 mg/mL) and soluble in 1 N HCl (50 mg/mL).
We also offer:
- Ampicillin Trihydrate, USP (A009)
- Ampicillin/Sulbactam (2:1) (A071)
- Ampicillin Anhydrous (A043)
- Ampicillin Sodium (A042)
- Ampicillin Trihydrate, EP (A020)
Ampicillin Trihydrate, USP conforms to United States Pharmacopoeia specifications.
|Mechanism of Action||Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.|
|Spectrum||Ampicillin, USP targets Gram-negative non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to Ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation.|
|Microbiology Applications||Ampicillin Trihydrate is often used to select for cells that have been transformed with a plasmid containing the ampR gene which confers resistance to Ampicillin.
Ampicillin Trihydrate can be used as a selective agent in several types of isolation media:
Aeromonas Medium Base - Ampicillin Selective Supplement
|Eukaryotic Cell Culture Applications||Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information, please visit our cell-culture database.|
|Solubility||Slightly soluble in water (10 mg/mL) and soluble in 1 N HCl (50 mg/mL)|
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