SKU: A019  / 
    CAS Number: 8063-24-9

    Acriflavine Hydrochloride

    $31.03 - $59.56

    Acriflavine Hydrochloride is a fluorescent dye commonly used as a topical contrast agent. It stains cell nuclei and is applied to tissues before miscrocopy.  Acroflavine has been used in confocal laser endomicroscopy.  It has also been used for strain selection.  Acriflavine is freely soluble in aqueous solution.

    This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.

    Mechanism of Action Acriflavine toxicity arises from its ability to bind to and intercalate DNA. DNA intercalation leads to numerous errors which have a lethal effect on targeted organisms.
    Microbiology Applications Acriflavine has been used in RNA fluorescent labeling applications by RNA hydrolysis using HCl.
    Cancer Applications Acriflavine has been shown to inhibit HIF-1, a heterodimeric transcription factor which responds to hypoxia and facilitates further cancer progress. Acriflavine prevents dimerization of HIF-1 to prevent its role in cancer growth.
    Molecular Formula C13H11N3 and C14H14N3 •HCl
    References

    Kawai M and Yamagishi J (2009)  Mechanisms of action of Acriflavine: Electron microscopic study of cell wall changes induced in Staphylococcus aureus by Acriflavine.  Microbiol. Immunol.  53(9):481-486  PMID 19703241 

    Lee K et al (2009)  Acriflavine inhibits HIF-1 dimerization, tumor growth, and vascularization.  Proc. Natl. Acad. Sci. USA 106 (42):17910-17915  PMID 19805192

    Levinson JW, Maher VM and McCormick JJ (1979)  Fluorescent labeling of fragments of high molecular weight RNA. Anal. Biochem. 93(2):399-406

     

    MIC Escherichia coli| 2 - 128|| Klebsiella pneumonia| 128 - 512|| Staphylococcus aureus| 3.13 - 50||