SKU: A014  / 
    CAS Number: 65710-07-8

    Apramycin Sulfate

    $68.00 - $266.00
    Apramycin Sulfate

    Apramycin Sulfate is an aminoglycoside antibiotic which binds the deep groove of RNA and effectively inhibits ribosomal translocation prohibiting protein synthesis. Apramycin was formerly called Nebramycin factor.  The compound is used in susceptibility testing applications.  Apramycin is sparingly soluble in water.  

    Mechanism of Action

    Apramycin Sulfate binds to the aminoacyl-transfer RNA site (A site) in the prokaryotic 30S ribosomal subunit.  This results in inhibition of ribosomal translocation thus an inhibition of protein synthesis.  

    The ribosome is a rich target for small-molecule antibiotics, and they use varied strategies to interfere with translation.
    Spectrum Apramycin Sulfate has activity against Gram-negative bacteria.
    Microbiology Applications Apramycin Sulfate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.  Representative MIC values include:
    • Escherichia coli 1 µg/mL － >512 µg/mL
    • Klebsiella pneumoniae 2 µg/mL － >256 µg/mL
    • For a representative list of Apramycin MIC values, click here.
    Molecular Formula C21H41N5O11 • H2SO4
    References

    Davis BD (1987)  Mechanism of bactericidal action of aminoglycosides.  Microbiol. Rev. 51(3): 341-350

    O'Connor S, Lam LKT, Jones ND, Chaney NO (1976)  Apramycin, a unique aminocyclitol antibiotic.  J. Org. Chem. 41(12):2087  

    Tsai A et al (2013)  The impact of aminoglycosides on the dynamics of translocation elongation. Cell Rep. 3(2):497-508 
    MIC Escherichia coli| 1 － >512|| Escherichia coli (ATCC 25922)| 4|| Haemophilus parasuis| 4 － 64|| Klebsiella pneumoniae| 2 － >256|| Pseudomonas aeruginosa| 4||