SKU: C008  / 
    CAS Number: 88040-23-7

    Cefepime

    $126.32 - $853.26

    Cefepime is a semi-synthetic, broad-spectrum, fourth-generation cephalosporin antibiotic that was patented in 1982 (Bristol-Myers Squibb).  Cefepime is bactericidal against a wide range of Gram-positive and Gram-negative bacteria, disrupting bacterial cell wall synthesis.  Cefepime is a zwitterion with a net neutral charge which crosses the outer membrane of Gram-negative bacilli more rapidly than other cephalosporins.  Cefepime appears to be intrinsically more resistant to hydrolysis by β-lactamases.  The compound is commonly used in antimicrobial susceptibility testing. 

    Cefepime is sparingly soluble in water. 

    We also offer:

    • Cefepime Hydrochloride (C009)
    Mechanism of Action Like β-lactams, cephalosporins interfere with penicillin binding protein (PBP) activity involved in the final phase of peptidoglycan synthesis. PBP’s catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid-encoded β-lactamases. Interestingly, cefepime is resistant to various β-lactamases encoded by otherwise resistant β-lactam bacteria strains.
    Spectrum Cefepime is a broad-spectrum antibiotic targeting a wide variety of naturally antibiotic resistant Gram-positive and Gram-negative bacteria. Some of these naturally resistant bacteria include Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae.
    Microbiology Applications Cefepime is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram -positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.
    • Pseudomonas aeruginosa 32 µg/mL – 256 µg/mL
    • Staphylococcus aureus 2 µg/mL – 16 µg/mL.
    • For a representative complete list of cefepime MIC values, click here.

    Cefepime was used in a MALDI-TOF MS-based direct-on-target microdroplet growth assay as part of a screening panel for rapid detection of ESBL, and AmpC β-Lactamases in Enterobacterales  (Correa-Martinez et al, 2019). 

    Cancer Research Applications Cefepime (50mM) was mixed with 8 different metal salts (copper, zinc, cobalt, nickel, cadmium, chromium, iron, and manganese ) in a 1:1 ratio and evaluated for proteasome-inhibitory and anti-proliferative properties using MDA-MB 231 human breast cancer cells. The complex with manganese had the highest inhibition of cell proliferation and proteasome activity. The complex induced apoptosis in a dose-and time-dependent manner and has the potential for use as an antitumor agent (Zhang et al, 2015).
    Molecular Formula C19H24N6O5S2
    References

    Georgopapadakou NH and Bertasso A (1993)  Mechanisms of Action of Cephalosporin 3'-quinolone Esters, Carbamates, and Tertiary Amines in Escherichia Coli.  Antimicrob. Agents. Chemother. 37(3):559-565

    Zhang Z, Bi C, Fan Y, Wang H and Bao Y (2015) Cefepime, a fourth-generation cephalosporin, in complex with manganese, inhibits proteasome activity and induces the apoptosis of human breast cancer cells. Int. J. Molec. Med. 36(4): 1143-1150

    Cefepime from TOKU-E:

    Correa-Martinez C, Idelevich EA, Sparbier K, Kostrzewa M, and Becker K (2019)  Rapid detection of extended-spectrum B-lactamases (ESBL) and AmpC B-Lactamases in Enterobacterales: Development of a screening panel using the MALDI-TOF MS-based direct-on-target microdroplet growth assay. Front. Microbiol. 10:13  PMID 30733710

    MIC Aeromonas spp.| 0.03 - 0.06|| Aeromonas spp. (ampicillin-resistant + cefazolin-resistant)| 0.03 - 8|| Agrobacterium radiobacter| 1 - 8|| Alcaligenes faecalis| 0.25 - >64|| Bacillus cereus (RK-75)| 62.5|| Bacillus dipsauri| 7.81|| Bacillus flexus| 125|| Bacillus sphaericus| 31.25|| Bacillus spp.| >32|| Bacteroides distasonis | 16 - >32|| Bacteroides fragilis | 32 - >64|| Bacteroides melaninogenicus | 16 - >32|| Bacteroides ovatus | 16 - >32|| Bacteroides septicum | 16 - >32|| Bacteroides thetaiotaomicron | 16 - >32|| Bordetella bronchiseptica| 0.25 - 64|| Brevibacillus agri| 125|| Brevundimonas vesicularis| 0.25 - 32|| Burkholderia cepacia| ≤0.06 - >256|| Burkholderia spp.| 4 - 32|| Campylobacter jejuni| 0.5 - 8|| Chryseobacterium indologenes| ≤0.06 - 16|| Chryseobacterium meningosepticum| 8 - 64|| Chryseomonas luteola| 31.25|| Citrobacter amalonaticus| ≤0.12 - 31.25|| Citrobacter braakii| ≤0.12 - >16|| Citrobacter diversus| 0.015 - >16|| Citrobacter farmeri| ≤0.12 - >16|| Citrobacter freundii| 0.015 - 64|| Citrobacter koseri| ≤0.06 - >16|| Citrobacter spp.| ≤0.12 - >16|| Clostridium perfringens| 0.5 - >16|| Comamonas acidovorans| 8 - >64|| Corynebacterium ammoniagenes| 7.81|| Corynebacterium cystitidis| 7.81|| Corynebacterium flavescens| 7.81|| Corynebacterium spp.| 0.06 - >32|| Enterobacter aerogenes| ≤0.06 - ≥64|| Enterobacter agglomerans| ≤0.25 - 0.5|| Enterobacter amnigenus| ≤0.12 - >16|| Enterobacter asburiae| ≤0.12 - >16|| Enterobacter cancerogenus| ≤0.12 - >16|| Enterobacter cloacae| 0.015 - ≥64|| Enterobacter gergoviae| ≤0.12 - >16|| Enterobacter hormaechei| ≤0.12 - >16|| Enterobacter intermedius| 0.12 - 31.25|| Enterobacter sakazakii| ≤0.12 - >16|| Enterobacter taylorae| ≤0.12 - >16|| Enterobacteriaceae| ≤0.12 - 64|| Enterococcus faecalis| 0.5 - >128|| Enterococcus faecium| ≤0.12 - >128|| Erwinia carotovora| 7.81|| Escherichia coli| ≤0.007 - 128|| Exiguobacterium acetylicum| 7.81|| Flavimonas oryzihabitans| 15.6|| Flavobacterium balustinum (HH299)| 31.25|| Haemophilus influenzae| 0.003 - 8|| Klebsiella ornithinolytica| 0.12 - >16|| Klebsiella oxytoca (ampicillin-resistant)| 0.03 - 8|| Klebsiella ozaenae| ≤0.12 - >16|| Klebsiella pneumonia| ≤0.007 - >256|| Klebsiella spp.| 0.015 - >16|| Klebsiella terrigena| ≤0.12 - >16|| Kocuria rosea| 31.25|| Listeria monocytogenes| 4 - 64|| Micrococcus spp.| 0.094 - 0.19|| Moraxella catarrhalis| ≤0.03 - 8|| Morganella morganii| 0.015 - >32|| Morganella spp.| ≤0.25 - 64|| Ochrobactrum anthropi| 8 - ≥128|| Oligella ureolytica| 0.25 - >64|| Paenibacillus apiarius| 62.5|| Pantoea agglomerans| 7.81|| Pasteurella multocida| <2|| Proteae spp.| ≤0.12 - >16|| Proteus mirabilis| 0.015 - >128|| Proteus rettgeri| ≤0.25 - >32|| Proteus spp.| ≤0.06 - 32|| Proteus vulgaris| 0.015 - 62.5|| Providencia rettgeri| 0.03 - 8|| Providencia spp.| ≤0.06 - 64|| Providencia stuartii| 0.03 - 4|| Pseudomonas aeruginosa| 0.06 - >256 || Pseudomonas cepacia (ampicillin-resistant + cefazolin-resistant)| 0.12 - 64|| Pseudomonas oryzihabitans| ≤0.06 - >64|| Pseudomonas stutzeri| ≤0.06 - >64|| Pseudomonas syringae| 31.25 - 62.5|| Psychrobacter immobilis| 15.6|| Ralstonia pickettii | 1 - 4|| Salmonella| ≤0.12 - 2|| Salmonella agona| ≤0.12 - 2|| Salmonella bareilly| ≤0.12 - 2|| Salmonella enterica| ≤0.12 - 2|| Salmonella enteritidis| ≤0.12 - 2|| Salmonella hadar| ≤0.12 - 2|| Salmonella heidelberg| ≤0.12 - 2|| Salmonella infantis| ≤0.12 - 2|| Salmonella litchfield| ≤0.12 - 2|| Salmonella Montevideo| ≤0.12 - 2|| Salmonella muenchen| ≤0.12 - 2|| Salmonella Newport| ≤0.12 - 2|| Salmonella panama| ≤0.12 - 2|| Salmonella Paratyphi| ≤0.12 - 2|| Salmonella schwarzengrund| ≤0.12 - 2|| Salmonella spp.| 0.015 - 0.5|| Salmonella stanley| ≤0.12 - 2|| Salmonella stpaul| ≤0.12 - 2|| Salmonella thompson| ≤0.12 - 2|| Salmonella typhi| 0.015 - 125|| Salmonella virchow| ≤0.12 - 2|| Serratia fonticola| ≤0.12 - >16|| Serratia liquefaciens| ≤0.12 - 62.5|| Serratia marcescens| 0.03 - 128|| Serratia odorifera| ≤0.12 - >16|| Serratia plymuthica| ≤0.12 - >16|| Serratia rubidaea| ≤0.12 - >16|| Shigella boydii| ≤0.12 - 1|| Shigella dysenteriae| ≤0.12 -15.6|| Shigella flexneri| ≤0.12 - 1|| Shigella sonnei| 0.015 - 1|| Shigella spp.| 0.015 - 1|| Sphingomonas spp.| 2 - 16|| Staphylococcus aureus| 0.125 - >128|| Staphylococcus auricularis (coagulase-negative + oxacillin-susceptible)| ≤0.12 - 8|| Staphylococcus capitis (coagulase-negative + oxacillin-susceptible)| ≤0.12 - 8|| Staphylococcus caprae (coagulase-negative + oxacillin-susceptible)| ≤0.12 - 8|| Staphylococcus cohnii| ≤0.12 - 7.81|| Staphylococcus epidermidis| 0.03 - 128|| Staphylococcus haemolyticus| ≤0.12 - 128|| Staphylococcus hominis| ≤0.12 - >128|| Staphylococcus intermedius (coagulase-negative + oxacillin-susceptible)| ≤0.12 - 8|| Staphylococcus lugdunensis (coagulase-negative + oxacillin-susceptible)| ≤0.12 - 8|| Staphylococcus mitis| 0.015 - 4|| Staphylococcus sanguis| ≤0.007 - 0.5|| Staphylococcus saprophyticus| ≤0.12 - >64|| Staphylococcus sciuri (coagulase-negative + oxacillin-susceptible)| ≤0.12 - 8|| Staphylococcus simulans| ≤0.12 - 16|| Staphylococcus spp. (coagulase-negative + oxacillin-susceptible)| ≤0.12 - 8|| Staphylococcus warneri (coagulase-negative + oxacillin-susceptible)| ≤0.12 - 8|| Stenotrophomonas maltophilia| 1 - 128|| Streptococcus agalactiae| 0.015 - 0.12|| Streptococcus dysgalactiae| ≤0.12 - 0.5|| Streptococcus equisimilis | ≤0.12 - 0.5|| Streptococcus pneumonia| ≤0.007 - >8|| Streptococcus pyogenes| 0.015 - 0.12|| Streptococcus spp.| ≤0.12 - 0.5|| Weeksella virosa | 0.5 - 16|| Xanthomonas maltophilia| 2 - >64|| Yersinia enterocolitica| 0.015 - 0.25||