Fosfomycin tromethamine is a broad-spectrum organophosphonate antibiotic with in vitro activity against most urinary tract pathogens. It is active against E. coli, Citrobacter,Enterobacter, Klebsiella, Serratia and Enterococcus.
|Mechanism of Action|
Fosfomycin tromethamine inhibits bacterial cell (peptidoglycan) synthesis. Specifically, it inhibits uridine diphospho-N-acetl-D-glucosamine enolpyruvyl transferase (MurA), thereby blocking the condensation of uridine diphosphate-N-acetylglucosamine, one of the first steps in peptidoglycan synthesis.
|Spectrum||Broad spectrum against both Gram-positive and Gram-negative pathogens|
|Eukaryotic Cell Culture Applications|
Investigators examined the effect of Fosfomycin on the immune system, specifically the in vitro function of human T cells, and was found to exert its action on the late stage of T-cell activation. It inhibited the proliferation of human lymphocytes induced by polyclonal T-cell mitogens. It suppressed mixed lymphocyte reaction and the production of interleukin-2 by T-cells. Fosfomycin also inhibited the expression of IL-2 receptor and transferrin receptor on T-cell surfaces. This property widens the therapeutic indices as it could be combined with immunosuppressants in organ transplantation (Morikawa et al, 1993).
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de Cueto M et al (2006) In vitro activity of Fosfomycin against extended-spectrum—lactamase producing Escherichia coli and Klebsiella pneumoniae: Comparison of susceptibility testing procedures. Antimicrob. Agents and Chemother 50:368-370
Falagas ME and Kastaris AC (2010) Fosfomycin for the treatment of multidrug-resistant, including extended-spectrum β-lactamase producing, Enterobacteriaceae infections: A systematic review. Lancet Infect. Dis. 10(1): 43-50 PMID 20129148
Grif K et al (2001) In vitro activity of Fosfomycin in combination with various antistaphylococcal substances. J Antimicrob. Chemother 48:209-217
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Morikawa K, Oseko F, Morikawa S, Sawada M(1993) Immunosuppressive activity of fosfomycin on human T-lymphocyte function in vitro. Antimicrob. Agents Chemother. 37(12): 2684-2687 PMID 7509146