Guanidine thiocyanate, Ultrapure, is a freely soluble chaotropic agent routinely used for DNA/RNA extraction protocols.
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|Mechanism of Action|
Extraction of cellular DNA and RNA releases DNases and RNases. Guanidine thiocyanate is used during cell lysis because of its ability to irreversibly inactivate degrading nucleases. In addition to its chaotropic properties, Guanidine thiocyanate has also shown to inactivate enveloped viruses and some non-enveloped viruses.
|Microbiology Applications||Guanidine thiocyanate is routinely used for DNA/RNA extraction protocols to prevent damage by degredative enzymes.|
|Eukaryotic Cell Culture Applications||Guanidine thiocyanate can be used to isolate RNA from both cell culture and tissue form human and animal sources. Authors extracted a protocol for extracting RNA from human placental tissue and from primary and transformed cell lines of human and animal origin, without any ultracentrifugation steps or phenol extractions. Human skin fibroblasts, primary hepatocytes from rats, and Fu5AH rat hepatoma cell line were used and the RNA extracted was intact, with good yield, iRNAse free and not contaminated by DNA. It was suitable for RT-PCR, Northern blotting, and in vitro protein translation (Zolfaghari et al, 1993).|
|Impurity Profile||Heavy Metal (as Pb): Not more than 5 ppm Iron: Not more than 5 ppm Sodium: Not more than 0.5% Barium: Not more than 5ppm Potassium: Not more than 0.005%|
Chomczynski P (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTech. 15(3):532-534, 536-537 PMID 7692896
Chomzynski P and Sacchi N 2006) The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: Twenty-something years on. Nat. Protoc. 1(2):581-585