Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. It inhibits protein synthesis in prokaryotes and eukaryotes. Hygromycin B is routinely used as a selection agent in transfection experiments to select eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).
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