SKU: J001  / 
    CAS Number: 16846-24-5

    Josamycin

    $75.60 - $294.00
    Josamycin is a macrolide antibiotic that inhibits protein synthesis.  It has anti-cancer properties, as it is able to overcome P-glycoprotein-dependent drug resistance in cancerous tumors.
    ApplicationMacrolide antibiotics such as Josamycin are useful in studying phagocytosis and cytokine production, immunomodulatory functions, and bacterial protein synthesis.
    Mechanism of ActionJosamycin inhibits peptide elongation on the ribosome by binding close to the peptidyltransferase center and blocking the peptide exit tunnel in the large ribosomal subunit. Synthesis of full-length proteins is completely shut down by Josamycin.
    Eukaryotic Cell Culture ApplicationsJosamycin was evaluated in cell culture with peritoneal cells and splenic cells from BALB/c mice. Phagocytic activity of C. albicans and cytokine production (IL12 and IL-18) by peritoneal cells and IL-4 production by splenic cells was evaluated, and results indicated that Josamycin acts as an immunosuppressive agent (Ortega et al, 2004).
    Cancer ApplicationsJosamycin was found to increase the accumulation of vinblastine and cyclosporine A in a dose-dependent manner in anticancer drug-resistant cells (P388/ADR). Since it inhibits the binding of vinblastine and cyclosporine A to P-glycoprotein, it may be used to overcome anticancer drug resistance. In fact, combination chemotherapy with macrolide antibiotics like Josamycin may overcome P-glycoprotein-dependent drug-resistant tumors (Wang et al, 2000).
    SolubilitySlightly soluble in ethanol, methanol and chloroform.
    SourceStreptomyces kitasatoensis and Streptomyces narbonensis var. josamyceticus.
    ReferencesLovmar M, Tenson T and Ehrenberg M (2004) Kinetics of macrolide action: The josamycin and erythromycin cases. J. Biol. Chem. 279(51):53506-53515 PMID 15385552 Ortega E et al (2004) Modificaiton of phagocytosis and cytokine production in peritoneal and splenic murine cells by erythromycin A, azithromycin and josamycin. J. Antimicrob. Chemother. 53(2):367-370. PMID 14729765 Wang L et al (2000) Reversal of anticancer drug resistance by macrolide antibiotics in vitro and in vivo. Clin. Exper. Pharmacol. Physiol. 27(8):587-593