SKU: N011  / 
    CAS Number: 389-08-2

    Nalidixic Acid

    $61.50 - $185.00
    Nalidixic Acid

    Nalidixic acid is a naphthyridone antibiotic similar in structure and mechanism to quinolones.

    TOKU-E offers two forms of nalidixic acid: nalidixic acid (N011) and nalidixic acid sodium salt, CulturePure® (N001). Nalidixic acid is sparingly soluble in aqueous solution at 0.1 mg/mL. Nalidixic acid sodium salt is freely soluble in aqueous solution at 50 mg/mL.

    Mechanism of ActionNalidixic acid indirectly inhibits proteins synthesis by binding DNA and interfering with enzymes involved in DNA replication and transcription.
    SpectrumNalidixic acid is a broad spectrum antibiotic commonly used against bacteria responsible for urinary tract infections.
    Microbiology ApplicationsNalidixic acid is commonly used in clinical in vitromicrobiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:
    • Escherichia coli 2 µg/mL - 8 µg/mL
    • Proteus mirabilis 2.1 µg/mL - 8 µg/mL
    • For a complete list of nalidixic acid MIC values, click here.
    Kiianitsa et al. used TOKU-E nalidixic acid at 100 µg/mL to study DNA gyrase inhibition in E. coli K-12. "Ultrasensitive isolation, identification and quantification of DNA–protein adducts by ELISA-based RADAR assay" 

    Media Supplements

    Nalidixic acid can be used as a selective agent in several types of isolation media:

    Columbia Blood Agar - Staphylococcus/Streptococcus selective supplement

    Wilkins-Chalgren Anaerobe Agar - Isolation of non-sporing anaerobes

    Wilkins-Chalgren Anaerobe Agar - Isolation of Gram-negative anaerobes

    Columbia Blood Agar - Gardnerella vaginalis Selective Supplement

    Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplement

    Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplements UVM I and UVM II

    Listeria Enrichment Broth - Modified Listeria Selective Enrichemnt Supplement

    Fraser Broth - Fraser Supplement

    Fraser Broth - Half Fraser Supplement

    Brucella Medium Base - Modified Brucella Selective Supplement

    Buffered Listeria Enrichment Broth - Listeria Selective Enrichment Supplement

    ChromogenicListeria Agar - ChromogenicListeria Selective Supplement

    ChromogenicListeria Agar - Chromogenic Listeria Differential Supplement

    Molecular FormulaC12H12N2O3
    Impurity ProfileResidue on Ignition: ≤0.2%
    Heavy Metals: ≤0.002%
    References

    Levitt, Martin, and et al. "Studies on the Mechanism of Action of Nalidixic Acid." Studies on the Mechanism of Action of Nalidixic Acid 4.4 (1973): 479-86. www.ncbi.gov. Web. 10 Sept. 2012.

    MICAeromonas spp.| ≥32|| Bacillus subtilis| ≥3.13|| Bacteroides fragilis| 2 － 64|| Bifidobacterium adolescentis| 62.5 － 125|| Bifidobacterium animalis| ≥500|| Bifidobacterium bifidum| 31.25 － 62.5|| Bifidobacterium breve| >500|| Bifidobacterium Infantis| ≥125|| Bifidobacterium longum| 62.5 － 125|| Bifidobacterium pseudolongum| 62.5 － 125|| Bifidobacterium sp.| 125 - 250|| Bifidobacterium thermophilum| ≥125|| Borrelia burgdorferi S.L.| 128 － >512|| Brucella suis (ATCC 27531)| ≥125|| Campylobacter jejuni| 2 － >256|| Campylobacter spp.| ≤1 － ≥64|| Citrobacter freundii| 4 － >32|| Citrobacter koseri| 2 － >32|| Clostridium perfringens| ≥8|| Clostridium spiroforme| ≥8|| Enterobacter aerogenes| 2 － >32|| Enterobacter cloacae| 2 － 512|| Enterobacteriaceae| 1 － 128|| Enterococcus faecalis| >32|| Enterococcus faecium| ≥32|| Escherichia coli| 1 － 256|| Haemophilus influenzae| 0.71 － 2|| Haemophilus spp.| 0.015 － 2|| Klebsiella pneumonia| 2 － >64|| Lactobacillus acidophilus| 250 － >500|| Lactobacillus bulgaricus| ≥500|| Lactobacillus casei| ≥500|| Lactobacillus lactis | ≥500|| Lactobacillus paracasei| ≥500|| Lactobacillus plantarum| ≥500|| Lactobacillus rhamnosus| ≥500|| Lactobacillus spp.| ≥500 －>1024|| Legionella pneumophila| 2|| Moraxella catarrhalis| 2 － 4|| Morganella morganii| 1 － >32|| Mycobacterium smegmatis| 3.125 － 50|| Neisseria spp.| 0.5 － 8|| Plesiomonas shigelloides| ≥32|| Proteus mirabilis| 4 － >32|| Proteus morganii | 1.56|| Proteus rettgeri | 1.56|| Proteus spp.| 4 － 4.1|| Proteus vulgaris| 1.56 － 4|| Providencia rettgeri| 4 － >32|| Providencia stuartii| 2 － >32|| Pseudomonas aeruginosa| <1 － >128|| Pseudomonas spp.| 32 － 128|| Salmonella enterica| 1 － >32|| Salmonella Enteritidis| 3.13|| Salmonella Paratyphi| 0.78|| Salmonella schottmulleri| 0.78|| Salmonella typhi| 4 - >184000|| Serratia marcescens| 1 － >32|| Shigella| 1 － 64|| Shigella flexneri| 0.12 － <4|| Shigella sonnei| 0.12 － 32|| Shigella spp.| 1.15 － 8|| Staphylococci| 16 － 128|| Staphylococcus aureus| >8 － >128|| Stenotrophomonas maltophilia| 8 － 32|| Streptococcus agalactiae| >32|| Streptococcus pneumonia| >128|| Streptococcus pyogenes| >32 － >100|| Treponema hyodysenteriae | ≥100|| Xylella fastidiosa| 16 － 32|| Yersinia enterocolitica| 4 － 8||