SKU: N001  / 
    CAS Number: 3374-05-8

    Nalidixic Acid Sodium, CulturePure®

    $45.62 - $109.37

    Nalidixic acid is a naphthyridone antibiotic similar in structure and mechanism to quinolones.

    TOKU-E offers two forms of nalidixic acid: nalidixic acid (N011) and nalidixic acid sodium salt, CulturePure® (N001). Nalidixic acid is sparingly soluble in aqueous solution at 0.1 mg/mL. Nalidixic acid sodium salt is freely soluble in aqueous solution at 50 mg/mL.

    Mechanism of ActionNalidixic acid indirectly inhibits proteins synthesis by binding DNA and interfering with enzymes involved in DNA replication and transcription.
    SpectrumNalidixic acid is a broad spectrum antibiotic commonly used against bacteria responsible for urinary tract infections.
    Microbiology ApplicationsNalidixic acid is commonly used in clinical in vitromicrobiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:
    • Escherichia coli 2 µg/mL - 8 µg/mL
    • Proteus mirabilis 2.1 µg/mL - 8 µg/mL
    • For a complete list of nalidixic acid MIC values, click here.

    Kiianitsa et al. used TOKU-E nalidixic acid at 100 µg/mL to study DNA gyrase inhibition in E. coli K-12. "Ultrasensitive isolation, identification and quantification of DNA–protein adducts by ELISA-based RADAR assay

    Media Supplements

    Nalidixic acid can be used as a selective agent in several types of isolation media:

    Columbia Blood Agar - Staphylococcus/Streptococcus selective supplement

    Wilkins-Chalgren Anaerobe Agar - Isolation of non-sporing anaerobes

    Wilkins-Chalgren Anaerobe Agar - Isolation of Gram-negative anaerobes

    Columbia Blood Agar - Gardnerella vaginalis Selective Supplement

    Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplement

    Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplements UVM I and UVM II

    Listeria Enrichment Broth - Modified Listeria Selective Enrichemnt Supplement

    Fraser Broth - Fraser Supplement

    Fraser Broth - Half Fraser Supplement

    Brucella Medium Base - Modified Brucella Selective Supplement

    Buffered Listeria Enrichment Broth - Listeria Selective Enrichment Supplement

    ChromogenicListeria Agar - ChromogenicListeria Selective Supplement

    ChromogenicListeria Agar - Chromogenic Listeria Differential Supplement

    Molecular FormulaC12H11N2NaO3
    References

    Levitt, Martin, and et al. "Studies on the Mechanism of Action of Nalidixic Acid." Studies on the Mechanism of Action of Nalidixic Acid 4.4 (1973): 479-86. www.ncbi.gov. Web. 10 Sept. 2012.

    MICAeromonas spp.| 32 - ?| 478| Bacillus subtilis (ATCC 6633)| 3.13 - ?| 1439| Bacteroides fragilis| 32 - 64| 401| Bacteroides fragilis (ATCC 25285)| 2 - ?| 650| Bacteroides fragilis (NCTC 9343)| 64 - ?| 401| Bifidobacterium adolescentis (ATCC 15704)| 62.5 - 125| 741| Bifidobacterium animalis (ATCC 27536)| 500 - ?| 741| Bifidobacterium bifidum (ATCC 15696)| 31.25 - 62.5| 741| Bifidobacterium bifidum (Bb12)| 62.5 - ?| 741| Bifidobacterium breve (ATCC 15700)| >500 - ?| 741| Bifidobacterium Infantis (ATCC 15697)| 125 - ?| 741| Bifidobacterium longum (ATCC 15707)| 62.5 - 125| 741| Bifidobacterium longum (ATCC 15708)| 62.5 - ?| 741| Bifidobacterium longum (RBL9)| 62.5 - 125| 741| Bifidobacterium pseudolongum (ATCC 25526)| 62.5 - 125| 741| Bifidobacterium sp. (RBL67)| 250 - ?| 741| Bifidobacterium sp. (RBL68)| 250 - ?| 741| Bifidobacterium sp. (RBL69)| 250 - ?| 741| Bifidobacterium sp. (RBL70)| 250 - ?| 741| Bifidobacterium sp. (RBL75)| 125 - ?| 741| Bifidobacterium sp. (RBL76)| 250 - ?| 741| Bifidobacterium thermophilum (ATCC 25866)| 125 - ?| 741| Borrelia burgdorferi S.L.| 128 - >512| 1129| Brucella suis (ATCC 27531)| 125 - ?| 741| Campylobacter jejuni| 2 - >32| 244| Campylobacter jejuni| ? - ?| 491| Campylobacter jejuni| ? - ?| 62| Campylobacter spp.| ≤1 - >=64| 829| Campylobacter spp.| 4 - ?| 478| Citrobacter freundii| 4 - >32| 244| Citrobacter koseri| 2 - >32| 244| Clostridium perfringens (ATCC 13124)| >8 - ?| 650| Clostridium spiroforme (A7)| >8 - ?| 650| Clostridium spiroforme (A8)| >8 - ?| 650| Clostridium spiroforme (B61)| >8 - ?| 650| Clostridium spiroforme (BA 1)| >8 - ?| 650| Clostridium spiroforme (C121Q)| 8 - ?| 650| Clostridium spiroforme (CED 1)| >8 - ?| 650| Clostridium spiroforme (D11)| >8 - ?| 650| Clostridium spiroforme (D13)| >8 - ?| 650| Clostridium spiroforme (D14)| >8 - ?| 650| Clostridium spiroforme (NCTC 11493)| >8 - ?| 650| Clostridium spiroforme (RPO 980)| >8 - ?| 650| Enterobacter aerogenes| 2 - >32| 244| Enterobacter aerogenes (ATCC 13048)| 3.13 - ?| 1439| Enterobacter cloacae| 2 - >32| 244| Enterobacter cloacae (963)| 6.25 - ?| 1439| Enterobacter cloacae (BW 1150)| ? - ?| 1392| Enterobacteriaceae| 1 - 128| 401| Enterococcus faecalis| >32 - ?| 244| Enterococcus faecalis (vancomycin-resistant)| >32 - ?| 244| Enterococcus faecium| >32 - ?| 244| Enterococcus faecium (vancomycin-resistant)| >32 - ?| 244| Escherichia coli| 1 - 256| 873| Escherichia coli (ATCC 25922)| 2 - ?| 650| Escherichia coli (ATCC 25922)| 4 - ?| 401| Escherichia coli (C316)| ? - >128| 1023| Escherichia coli (CA 8000)| 6.25 - ?| 792| Escherichia coli (chicken isolate)| ? - ?| 1037| Escherichia coli (enteroaggregative)| ? - ?| 491| Escherichia coli (enteroaggregative)| ? - ?| 62| Escherichia coli (enteroaggregative)| 128 - ?| 478| Escherichia coli (enterohaemorrhagic)| 1 - >32| 244| Escherichia coli (enterotoxigenic)| ? - ?| 62| Escherichia coli (enterotoxigenic)| ? - ?| 491| Escherichia coli (enterotoxigenic)| 256 - ?| 478| Escherichia coli (HB 101)| ? - ?| 1392| Escherichia coli (KAM32(pHPA))| 1 - ?| 1023| Escherichia coli (KAM32(pSTV28))| 1 - ?| 1023| Escherichia coli (KAM32(pSTVqepA))| 2 - ?| 1023| Escherichia coli (KAM32(pSTVΔqepA))| 1 - ?| 1023| Escherichia coli (KAM32)| 1 - ?| 1023| Escherichia coli (nalidixic acid-resistant)| >32 - ?| 244| Escherichia coli (nalidixic acid-susceptible)| 2 - >4| 244| Escherichia coli (NCTC 10418)| 2 - ?| 401| Escherichia coli (NIHJ JC-2 DNA gyrase)| 0.78 - ?| 392| Escherichia coli (NIHJ JC-2)| 6.25 - ?| 1439| Escherichia coli (NK 5819)| 1.56 - ?| 792| Escherichia coli (swine isolate)| ? - ?| 1037| Haemophilus influenzae| 0.71 - ?| 1439| Haemophilus influenzae (ATCC 49247)| 1 - ?| 401| Haemophilus spp.| 0.015 - 2| 401| Klebsiella pneumonia (G340)| 64 - ?| 174| Klebsiella pneumonia (G595)| 4 - ?| 174| Klebsiella pneumonia (GC 7535)| 4 - ?| 174| Klebsiella pneumonia (GC 7736)| >64 - ?| 174| Klebsiella pneumonia (GC 7737)| >64 - ?| 174| Klebsiella pneumonia (nalidixic acid-resistant)| >32 - ?| 244| Klebsiella pneumonia (nalidixic acid-susceptible)| 2 - >4| 244| Klebsiella pneumonia (penicillin-intermediate)| >32 - ?| 244| Klebsiella pneumonia (penicillin-resistant)| >32 - ?| 244| Klebsiella pneumonia (penicillin-susceptible)| >32 - ?| 244| Klebsiella pneumoniae (PCI-602)| 6.25 - ?| 1439| Lactobacillus acidophilus (P/N 601379 + oxgall-stressed)| >500 - ?| 740| Lactobacillus acidophilus (P/N 601379 + pH 2.0)| >500 - ?| 740| Lactobacillus acidophilus (P/N 601379)| >500 - ?| 740| Lactobacillus acidophilus (R052 + oxgall-stressed)| >500 - ?| 740| Lactobacillus acidophilus (R052 + pH 2.0)| >500 - ?| 740| Lactobacillus acidophilus (R052 + pH 4.0)| 250 - ?| 740| Lactobacillus acidophilus (R052)| 250 - ?| 740| Lactobacillus bulgaricus (P/N 601383 + oxgall-stressed)| >500 - ?| 740| Lactobacillus bulgaricus (P/N 601383 + pH 2.0)| >500 - ?| 740| Lactobacillus bulgaricus (P/N 601383 + pH 4.0)| 500 - ?| 740| Lactobacillus bulgaricus (P/N 601383)| >500 - ?| 740| Lactobacillus casei (R0215 + oxgall-stressed)| >500 - ?| 740| Lactobacillus casei (R0215 + pH 2.0)| >500 - ?| 740| Lactobacillus casei (R0215 + pH 4.0)| >500 - ?| 740| Lactobacillus casei (R0215)| >500 - ?| 740| Lactobacillus casei (R0256 + oxgall-stressed)| >500 - ?| 740| Lactobacillus casei (R0256 + pH 2.0)| >500 - ?| 740| Lactobacillus casei (R0256 + pH 4.0)| >500 - ?| 740| Lactobacillus casei (R0256)| >500 - ?| 740| Lactobacillus lactis (R0187 + oxgall-stressed)| >500 - ?| 740| Lactobacillus lactis (R0187 + pH 2.0)| >500 - ?| 740| Lactobacillus lactis (R0187 + pH 4.0)| >500 - ?| 740| Lactobacillus lactis (R0187)| >500 - ?| 740| Lactobacillus paracasei (P/N 601385 + oxgall-stressed)| >500 - ?| 740| Lactobacillus paracasei (P/N 601385 + pH 4.0)| >500 - ?| 740| Lactobacillus paracasei (P/N 601385)| >500 - ?| 740| Lactobacillus plantarum (P/N 601387 + oxgall-stressed)| >500 - ?| 740| Lactobacillus plantarum (P/N 601387 + pH 2.0)| >500 - ?| 740| Lactobacillus plantarum (P/N 601387 + pH 4.0)| 500 - ?| 740| Lactobacillus plantarum (P/N 601387)| >500 - ?| 740| Lactobacillus plantarum (R0202 + oxgall-stressed)| >500 - ?| 740| Lactobacillus plantarum (R0202 + pH 2.0)| >500 - ?| 740| Lactobacillus plantarum (R0202 + pH 4.0)| >500 - ?| 740| Lactobacillus plantarum (R0202)| >500 - ?| 740| Lactobacillus plantarum (R1078 + oxgall-stressed)| >500 - ?| 740| Lactobacillus plantarum (R1078 + pH 2.0)| >500 - ?| 740| Lactobacillus plantarum (R1078 + pH 4.0)| >500 - ?| 740| Lactobacillus plantarum (R1078)| >500 - ?| 740| Lactobacillus plantarum (R1096 + oxgall-stressed)| >500 - ?| 740| Lactobacillus plantarum (R1096 + pH 2.0)| >500 - ?| 740| Lactobacillus plantarum (R1096 + pH 4.0)| >500 - ?| 740| Lactobacillus plantarum (R1096)| >500 - ?| 740| Lactobacillus rhamnosus (R0011 + oxgall-stressed)| >500 - ?| 740| Lactobacillus rhamnosus (R0011 + pH 2.0)| >500 - ?| 740| Lactobacillus rhamnosus (R0011 + pH 4.0)| >500 - ?| 740| Lactobacillus rhamnosus (R0011)| >500 - ?| 740| Lactobacillus rhamnosus (R1039 + oxgall-stressed)| >500 - ?| 740| Lactobacillus rhamnosus (R1039 + pH 2.0)| >500 - ?| 740| Lactobacillus rhamnosus (R1039 + pH 4.0)| >500 - ?| 740| Lactobacillus rhamnosus (R1039)| >500 - ?| 740| Lactobacillus spp.| >1024 - ?| 1401| Lactobacillus spp. (L09)| ≥500 - ?| 908| Lactobacillus spp. (L36)| ≥500 - ?| 908| Lactobacillus spp. (L44)| ≥500 - ?| 908| Lactobacillus spp. (L48)| ≥500 - ?| 908| Lactobacillus spp. (L49)| ≥500 - ?| 908| Legionella pneumophila| 2 - ?| 244| Morganella morganii| 1 - >32| 244| Mycobacterium smegmatis (CSLMC2 205)| 25 - ?| 792| Mycobacterium smegmatis (CSMC2 105)| 50 - ?| 792| Mycobacterium smegmatis (mc2155)| 50 - ?| 792| Mycobacterium smegmatis (MDR-40)| 50 - ?| 792| Mycobacterium smegmatis (MDR-75)| 3.125 - ?| 792| Mycobacterium smegmatis (MSR101)| 6.25 - ?| 792| Mycobacterium smegmatis (MSR102)| 50 - ?| 792| Neisseria spp.| 0.5 - 8| 401| Plesiomonas shigelloides| 32 - ?| 478| Proteus mirabilis (IFO 3849)| 12.5 - ?| 1439| Proteus mirabilis (nalidixic acid-resistant)| >32 - ?| 244| Proteus mirabilis (nalidixic acid-susceptible)| 4 - >4| 244| Proteus morganii (IFO 3848)| 1.56 - ?| 1439| Proteus rettgeri (IFO 3850)| 1.56 - ?| 1439| Proteus spp. (indole-negative)| 4 - ?| 1439| Proteus spp. (indole-positive)| 4.1 - ?| 1439| Proteus vulgaris (HX-19)| 1.56 - ?| 1439| Proteus vulgaris (OX-19)| 3.13 - ?| 1439| Providencia rettgeri| 4 - >32| 244| Providencia stuartii| 2 - >32| 244| Pseudomonas aeruginosa (ATCC 27853)| >128 - ?| 401| Pseudomonas aeruginosa (ciprofloxacin-resistant)| 4 - >32| 244| Pseudomonas aeruginosa (ciprofloxacin-susceptible)| 4 - >32| 244| Pseudomonas aeruginosa (IFO 3445)| >100 - ?| 1439| Pseudomonas aeruginosa (IID1210)| >100 - ?| 1439| Pseudomonas aeruginosa (NCTC 10490)| 25 - ?| 1439| Pseudomonas aeruginosa (NCTC 10662)| >128 - ?| 401| Pseudomonas aeruginosa (OHD)| <1 - ?| 913| Pseudomonas aeruginosa (PAO)| 256 - ?| 913| Pseudomonas spp.| 32 - 128| 401| Salmonella enterica| 1 - >32| 244| Salmonella Enteritidis (G14)| 3.13 - ?| 1439| Salmonella Paratyphi (1015)| 0.78 - ?| 1439| Salmonella schottmulleri (8006)| 0.78 - ?| 1439| Salmonella spp.| ? - ?| 491| Salmonella spp.| ? - ?| 62| Salmonella spp.| 2.55 - ?| 1439| Salmonella spp.| 16 - ?| 478| Salmonella typhi (901)| 6.25 - ?| 1439| Salmonella typhi (Various)| >184000 - ?| 1122| Salmonella typhimurium (IID971)| 6.25 - ?| 1439| Salmonella typhimurium (L133)| 4 - ?| 1008| Salmonella typhimurium (LX1054)| 16000 - ?| 1122| Salmonella typhimurium (SL1344)| 4 - ?| 1008| Salmonella typhimurium (T39)| 16000 - ?| 1122| Serratia marcescens| 1 - >32| 244| Serratia marcescens| 2.65 - ?| 1439| Serratia marcescens (IAMI1184)| 6.25 - ?| 1439| Serratia marcescens (UD60)| 6.25 - ?| 1439| Shigella| 1 - 64| 1429| Shigella flexneri| 0.12 - 0.5| 1085| Shigella flexneri| ? - ?| 62| Shigella flexneri| ? - ?| 491| Shigella sonnei| 0.12 - 32| 1085| Shigella sonnei| ? - ?| 491| Shigella sonnei| ? - ?| 62| Shigella spp.| 1.15 - ?| 1439| Shigella spp.| 8 - ?| 478| Staphylococci| 16 - 128| 401| Staphylococcus aureus| >32 - ?| 244| Staphylococcus aureus (ATCC 25923)| >8 - ?| 650| Staphylococcus aureus (ATCC 25923)| 128 - ?| 401| Staphylococcus aureus (ATCC 29213)| 128 - ?| 401| Staphylococcus aureus (ATCC 6571)| >128 - ?| 401| Staphylococcus aureus (E-46)| >100 - ?| 1439| Staphylococcus aureus (FAD 209P JC-1)| >100 - ?| 1439| Staphylococcus aureus (methicillin-resistant)| >32 - ?| 244| Staphylococcus aureus (MS5935 topoisomerase IV)| 25 - ?| 392| Staphylococcus aureus (Terajima)| 25 - ?| 1439| Stenotrophomonas maltophilia| ? - ?| 198| Streptococcus agalactiae| >32 - ?| 244| Streptococcus pneumonia (ATCC 49619)| >128 - ?| 401| Streptococcus pyogenes| >32 - ?| 244| Streptococcus pyogenes| >100 - ?| 1439| Streptococcus pyogenes (IID692)| >100 - ?| 1439| Treponema hyodysenteriae| 100 - >100| 1427| Xylella fastidiosa (187)| 32 - ?| 628| Xylella fastidiosa (237)| 16 - ?| 628| Xylella fastidiosa (276)| 16 - ?| 628| Xylella fastidiosa (Ann1)| 32 - ?| 628| Xylella fastidiosa (AO5)| 32 - ?| 628| Xylella fastidiosa (B02)| 32 - ?| 628| Xylella fastidiosa (Dixon)| 32 - ?| 628| Xylella fastidiosa (IO3)| 32 - ?| 628| Xylella fastidiosa (Temecula)| 32 - ?| 628| Xylella fastidiosa (Texas)| 16 - ?| 628| Yersinia enterocolitica| ? - ?| 62| Yersinia enterocolitica| ? - ?| 491|