Nitrocefin is a chromogenic cephalosporin used to detect β-lactamases produced by β-lactam resistant bacteria. It does not appear to have antimicrobial properties, but has useful diagnostic properties. It can be used as a reagent in β-lactamase activity studies. Nitrocefin is soluble in DMSO.
This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for details.
|Application||Nitrocefin is useful for the detection of β-lactamase patterns from bacterial cell extracts by isoelectric focusing. It can also be used in biosensor development and protein switch technology.|
|Mechanism of Action||β-lactamases hydrolyze the amide bond between the carbonyl carbon and the nitrogen in the β-lactam ring of Nitrocefin. Intact Nitrocefin is converted to hydrolyzed Nitrocefin under visible light (~ 380-500 nm) and a color change occurs from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm).|
|Microbiology Applications||Nitrocefin is used to detect β-lactamase activity from suspected β-lactam resistant bacteria (see Protocol). It is commonly used at a working concentration of 0.5 - 1.0 mg/ml.|
Nitrocefin (TOKU-E) was used to study:
Parr, TR et al (1984) Simple screening method for beta-lactamase-positive and-negative Ampicillin-resistant Haemophilus Influenzae isolates. J. Clin. Microbiol. 20(1):131-132