Piperacillin Sodium Salt, USP is the sodium salt form of Piperacillin, a semi-synthetic, extended spectrum fungistatic β-lactam fourth-generation ureidopenicillin. Derived from Ampicillin, Piperacillin was developed by Toyama Chemical Co, Tokyo, Japan. It has a broad spectrum activity aginsat Gram-positive and Gram-negative aerobic and anaerobic bacteria. It has greater activiity agasint β-lactamase-producing organisms compared ot the other penicillins, however is is styll hydrolysed by plasmid-mediated β-lactamases TEM-1). It can also be used to study multidrug resistance.
Piperacillin Sodium Salt, USP is freely soluble in aqueous solution.
We also offer:
- Piperacillin (P017)
|Mechanism of Action
|β-lactams interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to β-lactams is commonly due to cells containing plasmid-encoded β-lactamases.
|Piperacillin is a broad-spectrum antibiotic targeting a wide range of susceptible Gram-positive and Gram-negative bacteria. It targets both aerobic and anaerobic bacteria. It can be used against Enterococcus species, Pseudomonas aeruginosa, and E. coli.
Piperacillin Related Compound A: ≤ 3.5%
Piperacillin Related Compound C: ≤ 1.0%
|Piperacillin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. It is commonly used in a mixture with the beta-lactamase inhibitor Tazobactam which enhances is effectiveness. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC ranges include:
For a representative list of Piperacillin MIC values, click here.
THe MIC of Piperacilin for most strains, apart form B-lactamase strains, is little affected by increases in inoculum size up to 106 organisms/ml. Piperacillin activity decreased with increasing pH, and Mueller Hinton broth produced the most consistent results for most orgnaisms.
|Plant Biology Applications
|Piperacillin was effective in removing background contamination in Agrobacterium-mediated plant transformation, effective against Agrobacterium tumefaciens strains LBA4404 and EHA101 in plant tissue culture experiments (Ogawa and Mii, 2004).
Holmes B, Richards DM, Brogden RN and Heel RC (1984) Piperacillin. Drugs 28:375–425 PMID 6391888
Ida K, Hirata S, Nakamuta S and Koike M (1978) Inhibition of cell division of Escherichia coli by a new synthetic penicillin, Piperacillin. Antimicrob. Agents Chemother. 14 (2) 257-266
Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in Gram-negative bacilli. Am. J. Med. 103:51
Karlowsky J et al (2003) Comparison of four antimicrobial susceptibility testing methods to determine the in vitro activities of Piperacillin and Piperacillin-Tazobactam against clinical isolates of Enterobacteriaceae and Pseudomonas aeruginosa. J. Clin. Microbiol. 41(7):3339-3343