SKU: C011  / 
    CAS Number: 64485-93-4

    Cefotaxime Sodium, USP

    $100.25 - $428.87

    Cefotaxime Sodium, USP is broad-spectrum, third-generation cephalosporin. It interferes with bacterial peptidoglycan synthesis. Cefotaxime sodium is freely soluble in aqueous solution. 

    TOKU-E offers two forms of cefotaxime sodium:

    • Cefotaxime Sodium, USP (C011)
    • Cefotaxime Sodium, CulturePure® (C106)

    Cefotaxime Sodium, USP conforms to United States Pharmacopoeia specifications.

    Mechanism of Action Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Cefotaxime Sodium however, is largely resistant to β-lactamases.

     

    Spectrum Cefotaxime Sodium has broad-spectrum activity against a wide variety of Gram-positive and Gram-negative bacteria. However, unlike many cephalosporin antibiotics, cefotaxime sodium is not effective against Pseudomonas aeruginosa.
    Microbiology Applications

    Cefotaxime Sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.  Representative MIC values include:

      • Neisseria gonorrhoeae 0.008 µg/mL – 0.03 µg/mL
      • Streptococcus pneumoniae ≤0.007 µg/mL - 0.25 µg/mL
      • For a complete list of Cefotaxime MIC values, click here.
    Plant Biology Applications

    Cefotaxime is often used in Agrobacterium tumefaciens mediated transformations to combat bacterial growth. It is also sometimes used in combination with Vancomycin as it has a synergistic effect.   

    Molecular Formula C16H16N5NaO7S2
    Impurities Chromatographic Purity:
    Single Impurity: ≤1.0%
    Total Impurities: ≤3.0%
    References

    General:

    Georgopapadakou NH (1992)  Mechanisms of action of cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli. Antimicrob. Agents. Chemother.  37(3): 559-65

    Mathias RJ and Boyd LA (1986)  Cefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat (Triticum aestivum L. em Thell). Plant Sci. 46:217-223

    Cefotaxime Sodium from TOKU-E:

    Ferrer-González E, Kaul M, Parhi AK, LaVoie EJ and Pilch DS (2017) Lactam antibiotics with a high affinity for PBP2 act synergistically with the FtsZ-targeting agent TXA707 against methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 61(9):e00863-17

    Lefurgy ST et al (2016)  Analysis of the structure and function of FOX-4 cephamycinase. Antimicrob. Agents. Chemother.