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Detailed Description
CAS Number: 66258-76-2
Molecular Formula: C23H27N5O7S ∙ H20
Molecular Weight: 535.57
Mechanism of Action: β-lactams interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to β-lactams is commonly due to cells containing plasmid-encoded β-lactamases.
Storage Conditions: 2-8 °C
Tariff Code: 2941.10.5000
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Applications
Spectrum: Piperacillin is broad-spectrum, targeting a wide range of susceptible Gram-positive and Gram-negative bacteria. It targets both aerobic and anaerobic bacteria. It can be used against Enterococcus species, Pseudomonas aeruginosa, and E. coli.
Microbiology Applications: Piperacillin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram- positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. It is commonly used in a mixture with the β-lactamase inhibitor Tazobactam which enhances is effectiveness. Representative MIC values include:
- Neisseria spp. 0.015 µg/mL – 32 µg/mL
- Bacteroides fragilis 0.25 µg/mL - 32 µg/mL
For a representative list of Piperacillin MIC values, click here.
Plant Biology Applications: In vitro activity of Piperacillin against Agrobacterium tumefaciens strains LBA4404 and EHA101 was examined and found to be effective in eliminating the bacteria involved in Agrobacterium-mediated plant genetic transformation (Ogawa and Mii, 2004).
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Specifications
Form: Powder
Appearance: White crystalline powder
Source: Semi-synthetic
Water Content (Karl Fischer): 2.0-4.0%
pH: 2.0-4.5
Assay: (on dried basis): 96.0-103.0%
Heavy Metals: ≤ 0.002%
Optical Rotation: +155° to +175°
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Technical Data
Solubility: Sparingly soluble in aqueous solution
Impurity Profile: Related Substances:
Impurity A: ≤ 1.0%
Impurity B: ≤ 0.2%
Impurity C: ≤ 0.4%
Impurity D: ≤ 2.0%
Ampicillin: ≤ 0.2% -
References
References:
Ogawa Y and Mii M (2004) Screening for highly active beta-lactam antibiotics against Agrobacterium tumefaciens. Arch. Microbiol. 181:331-336
Pitout JD, Sanders CC, and Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in Gram-negative bacilli. Am. J. Med. 103:51
Piperacillin is a semi-synthetic, extended spectrum fungistatic β-lactam fourth-generation ureidopenicillin. It was developed by Toyama Chemical Co, Tokyo, Japan and is derived from Ampicillin. Piperacillin is commonly used in combination with the β-lactamase inhibitor Tazobactam. It can be used to study multidrug resistance.
Piperacillin is formulated as the monohydrate and is sparingly soluble in aqueous solution.
We also offer:
- Piperacillin Sodium (P010)
CAS Number: 66258-76-2
Molecular Formula: C23H27N5O7S ∙ H20
Molecular Weight: 535.57
Mechanism of Action: β-lactams interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to β-lactams is commonly due to cells containing plasmid-encoded β-lactamases.
Storage Conditions: 2-8 °C
Tariff Code: 2941.10.5000
Spectrum: Piperacillin is broad-spectrum, targeting a wide range of susceptible Gram-positive and Gram-negative bacteria. It targets both aerobic and anaerobic bacteria. It can be used against Enterococcus species, Pseudomonas aeruginosa, and E. coli.
Microbiology Applications: Piperacillin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram- positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. It is commonly used in a mixture with the β-lactamase inhibitor Tazobactam which enhances is effectiveness. Representative MIC values include:
- Neisseria spp. 0.015 µg/mL – 32 µg/mL
- Bacteroides fragilis 0.25 µg/mL - 32 µg/mL
For a representative list of Piperacillin MIC values, click here.
Plant Biology Applications: In vitro activity of Piperacillin against Agrobacterium tumefaciens strains LBA4404 and EHA101 was examined and found to be effective in eliminating the bacteria involved in Agrobacterium-mediated plant genetic transformation (Ogawa and Mii, 2004).
Form: Powder
Appearance: White crystalline powder
Source: Semi-synthetic
Water Content (Karl Fischer): 2.0-4.0%
pH: 2.0-4.5
Assay: (on dried basis): 96.0-103.0%
Heavy Metals: ≤ 0.002%
Optical Rotation: +155° to +175°
Solubility: Sparingly soluble in aqueous solution
Impurity Profile: Related Substances:
Impurity A: ≤ 1.0%
Impurity B: ≤ 0.2%
Impurity C: ≤ 0.4%
Impurity D: ≤ 2.0%
Ampicillin: ≤ 0.2%
References:
Ogawa Y and Mii M (2004) Screening for highly active beta-lactam antibiotics against Agrobacterium tumefaciens. Arch. Microbiol. 181:331-336
Pitout JD, Sanders CC, and Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in Gram-negative bacilli. Am. J. Med. 103:51
