Tetracycline, USP is a light-sensitive bacteriostatic polyketide antibiotic frequently used in a wide range of in-vitro cell culture applications. Tetracycline is a first-generation Tetracycline antibiotic and was first identified in 1953 by Chemist Lloyd Conover’s team at Pfizer, in collaboration with R.B. Woodward of Harvard University. Tetracycline is a naturally occurring antibiotic from S. aureofaciens, S. rimosus, and S. viridofacien that shows wide ranging activity against both Gram-negative and Gram-positive bacteria.
Tetracycline is a protein synthesis inhibitor. Tetracycline bind the 30s ribosomal subunit, preventing the aminoacyl-tRNA from attaching to the A site. Consequently, protein synthesis is inhibited. Resistance to tetracycline arises from loss of cell wall permeability, tetracycline efflux, ribosome protection and tetracycline modification.
Tetracycline is used to study transcriptional activation. Knowledge of Tetracycline led to the development of a popular inducible expression system in eukaryotic cells known as Tet-Off and Tet-On. Tetracycline is also used in multidrug resistance studies and in cell culture applications as a selective agent. Additionally, it promotes expression of the P450 proteins.
Tetracycline, USP is sparingly soluble in aqueous solution.
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Tetracycline, USP conforms to United States Pharmcopeia specifications.
