Tylosin A, EvoPure®is a highly purified (>99.00% pure) form of tylosin A. Tylosin is a macrolide antibiotic that was originally isolated from Streptomyces fradiae by the Lilly Research Laboratories in 1961 from a soil sample collected in Thailand. Tylosin is a mixture of four macrolide antibiotics, the main component of the mixture (> 80%) is tylosin A; tylosin B (desmycosin), tylosin C (macrocin), and tylosin D (relomycin) may also be present. Tylosin has broad-spectrum bacteriostatic activity against Gram-positive bacteria and mycoplasma, but much less activity against most Gram-negative bacteria and fungi.
Tylosin A contains a polyketide lactone (tylactone) substituted with three 6-deoxyhexose sugars. The addition of D-mycaminose to the aglycone is followed by concurrent ring oxidation at C-20 and C-23 (to generate the tylonolide moiety) and substitution with L-mycarose and 6-deoxy-Dallose.
Tylosin, like other Macrolide antibiotics, is a bacteriostatic compound that reversibly bind to the 23S rRNA in the 50S (L27 protein) ribosome subunit and inhibit mRNA-directed protein synthesis.
Tylosin has been used to study protein synthesis, abscess prevention in cattle, and Mycoplasma infections.
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|Application||Tylosin A, EvoPure® can be used as a reference standard and to study tylosin A as an individual compound.|
|Mechanism of Action||Tylosin A binds to the 50S ribosomal subunit interfering with the binding of aminoacyl-tRNA-to 50S and block peptide bond formation. This inhibits the translocation of a newly synthesized peptidyl tRNA molecule from the acceptor site to the peptidyl site.|
Loke, M. L., and F. Ingerslev. "Stability of Tylosin A in Manure Containing Test Systems Determined by High Performance Liquid Chromatography." Chemosphere 40.7 (2000): n. pag. Nih.gov. Web. 27 Dec. 2013.
Thompson, T. S., S. F. Pernal, and D. K. Noot. "Degradation of Incurred Tylosin to Desmycosin--implications for Residue Analysis of Honey." Analytica Chimica 586 (2007): 304-11. Nih.gov. Web. 27 Dec. 2013.