4-Epitetracycline Ammonium Salt, EvoPure is a highly purified version of 4-Epitetracycline, an epimer of Tetracycline. Tetracycline is a broad-spectrum antibacterial and selection agent.
We also offer:
- 4-Epitetracycline Hydrochloride, EvoPure (T053)
| Mechanism of Action | Under acid conditions, Tetracycline undergoes a reversible epimerization at the fourth carbon, producing a mixture of tetracycline and 4-Epitetracycline. Tetracycline inhibits the growth of Gram-positive and Gram-negative bacteria by disrupting codon-anticodon interactions at the ribosome, thus blocking protein synthesis. Specifically, Tetracycline binds to the 30S ribosomal subunit and blocks the attachment of charged aminoacyl-tRNA to the A site on the ribosome, preventing peptide elongation. |
| Spectrum | Effective against Gram-positive and Gram-negative bacteria, Mycoplasma. |
| Microbiology Applications |
4-Epitetracycline Ammonium Salt, EvoPure can be use as an analytical standard. |
| Molecular Formula | C22H27N3O8 |
| References |
Fernandez AA, Noceda VT, Carrera ES (1969) Simultaneous separation and quantitative determination of tetracycline, anhydrotetracycline, 4-Epitetracycline, and 4-Epi-anhydrotetracycline in degraded tetracyclines by thin-layer chromatography. J. Pharm. Sci. 58(4):443-446 Mazur X, Eppenberger HM, Bailey JE and FUssenegger M (2000) A novel autoregulated proliferation-controlled production process using recombinant CHO cells. Biotechnol and Bioeng. 65(2):144-150 |
