SKU: C086  / 
    CAS Number: 67-48-1

    Choline Chloride, USP

    $27.19 - $38.32

    Choline Chloride is an essential nutrient commonly grouped with the B complex vitamins that plays key roles in many biological processes.  Choline is found in sphingomyeline and lecithin.  It is a precursor to acetylcholine.  It plays a role in methyl group metabolism and can be used as a standard in methionine and choline metabolism. 

    Choline Chloride is soluble in aqueous solution.

    Mechanism of Action Choline serves as a precursor component to two major cell membrane components: phosphatidylcholine and sphingomyelin. In addition, choline also functions as a methyl group donor to several different substrates.
    Microbiology Applications Because of its role in normal cellular processes, Choline Chloride can be used as a cell culture media supplement.
    Eukaryotic Cell Culture Applications Choline was added to culture medium of in vitro-produced embryos to see if it altered the phenotype of blastocysts.  Choline was found to alter the phenotype of preimplantation bovine embryos produced in vitro.  Choline chlorine can act in a concentraiton-dependent manner to alter development, gene expression, and DNA methylation (ref).
    Plant Biology Choline chloride based solvents can be used in extraction media for extracting phenolic compounds.
    Impurities Arsenic: ≤2ppm
    Lead: ≤0.3ppm
    Limit of Amines: ≤0.001%
    Individual Impurity: ≤0.3%
    Total Impurities: ≤2.0%
    Organic Volatile Impurities: Meets requirements
    References

    Brailoiu E et al (2019)  Choline is an intracellular messenger linking extracellular stimuli to IP3-evoked Ca2+ signals through sigma-1 receptors.  Cell Rep. 26(2):P330-337 

    Estrada-Cortés E et al (2020)  Effects of choline on the phenotype of the cultured bovine preimplantation embryo. J. Dairy Sci. 103(11):10784-10796  PMID 32896407

    Razboršek M et al (2020)  Choline Chloride based natural deep eutectic solvents as extraction media for extracting phenolic compounds from chokeberry (Aronia Melanocarpa). Molecules. 25(7):1619 PMID 32244757

    Xu H et al (2021)  Comprehensive analysis of important parameters of choline chloride-based deep eutectic solvent pretreatment of lignocellulosic biomass.  Bioresour Technol 319:124209