Choline Chloride is an essential nutrient commonly grouped with the B complex vitamins that plays key roles in many biological processes. Choline is found in sphingomyeline and lecithin. It is a precursor to acetylcholine. It plays a role in methyl group metabolism and can be used as a standard in methionine and choline metabolism.
Choline Chloride is soluble in aqueous solution.
|Mechanism of Action
|Choline serves as a precursor component to two major cell membrane components: phosphatidylcholine and sphingomyelin. In addition, choline also functions as a methyl group donor to several different substrates.
|Because of its role in normal cellular processes, Choline Chloride can be used as a cell culture media supplement.
|Eukaryotic Cell Culture Applications
|Choline was added to culture medium of in vitro-produced embryos to see if it altered the phenotype of blastocysts. Choline was found to alter the phenotype of preimplantation bovine embryos produced in vitro. Choline chlorine can act in a concentraiton-dependent manner to alter development, gene expression, and DNA methylation (ref).
|Choline chloride based solvents can be used in extraction media for extracting phenolic compounds.
Limit of Amines: ≤0.001%
Individual Impurity: ≤0.3%
Total Impurities: ≤2.0%
Organic Volatile Impurities: Meets requirements
Brailoiu E et al (2019) Choline is an intracellular messenger linking extracellular stimuli to IP3-evoked Ca2+ signals through sigma-1 receptors. Cell Rep. 26(2):P330-337
Estrada-Cortés E et al (2020) Effects of choline on the phenotype of the cultured bovine preimplantation embryo. J. Dairy Sci. 103(11):10784-10796 PMID 32896407
Razboršek M et al (2020) Choline Chloride based natural deep eutectic solvents as extraction media for extracting phenolic compounds from chokeberry (Aronia Melanocarpa). Molecules. 25(7):1619 PMID 32244757
Xu H et al (2021) Comprehensive analysis of important parameters of choline chloride-based deep eutectic solvent pretreatment of lignocellulosic biomass. Bioresour Technol 319:124209