Danofloxacin Mesylate is a synthetic, broad-spectrum fluoroquinolone with antibacterial activity. It is used in veterinary medical research applications. It is used to combat the organisms causing respiratory disease in cattle, chickens, and pigs.
| Mechanism of Action | Fluoroquinolone antibiotics target bacterial DNA gyrase (topoisomerase type II), an enzyme which reduces DNA strain during replication and is essential for DNA packaging, transcription, and replication. Thus, DNA synthesis and cell division is inhibited. |
| Spectrum | Broad-spectrum, including Gram-positive and Gram-negative bacteria. Also effective for Mycoplasmas. Representative genera include Actinobacillus, Escherichia, Klebsiella, Mannheima, Salmonella, and Staphylococcus. |
| Microbiology Applications | Danofloxacin is effective for cattle pathogens such as Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni. Danofloxacin is highly active in vitro against Mycoplasma species that cause respiratory infections in cattle, pigs, and poultry (Cooper et al, 1993). Representative MIC values include: o Mycoplasma species 0.008 µg/mL – 0.5 µg/mL o Pasteurella mulltocida 0.00375-0.125 ug/ml o For a complete list of Danofloxacin MIC values, click here |
| Eukaryotic Cell Culture Applications | The potential effect of Danofloxacin on oxidative stress was examined in vitro. Danofloxacin did not induce oxidative stress in LLC-PK1 cells during exposure of ≤400 μM for 24 hours. Assessment involved reviewing reactive oxygen species (ROS) level, lipid peroxidation, cell apoptosis and antioxidative enzyme activities. Authors note noted an increased activity of antioxidant enzymes (superoxide dismutase and catalase). (Yue et al, 2013). |
| Molecular Formula | C19H20FN3O3 • CH4O3S |
| References | Cooper AC, Fuller JR, Fuller MK, Whittlestone P, and Wise DR (1993) In vitro activity of danofloxacin, tylosin and oxytetracycline against Mycoplasmas of veterinary importance. Res. Vet. Sci 54(3):329-334 PMID 8393208 Kawai K et al (2013) Antimicrobial susceptibilities of Mycoplasma isolated from bovine mastitis in Japan. Animal Sci. J 85(1):96-99 Raemdonck DL, Tanner AC, Tolling ST and Michener SL (1992) In vitro susceptibility of avian Escherichia coli and Pasteurella multocida to danoflxacin and fie other antimicrobials. Avian Dis. 36(4):964-967 PMID 1336664 Yu CH et al (2013) Effect of danofloxacin on reactive oxygen species production, lipid peroxidation, and antioxidant enzyme activities in kidney tubular epithelial cell line, LLC-PK1. Basic. Clin. Pharmacol. Toxicol. 113(6):377-384. PMID 23855763 |
