SKU: D022  / 
    CAS Number: N/A

    De-Plasma™

    $127.86 - $263.18

    De-Plasma™ is a potent and safe anti-Mycoplasma reagent which can be used to eliminate Mycoplasma contamination or can be used prophylactically to prevent contamination.  It consists of a proprietary mixture of three antimicrobial compounds with anti-Mycoplasma properties. 

    Mycoplasma belong to a unique genus of bacteria that lack cell walls.  Their small size and ubiquitous distribution account for their ability to go undetected in seemingly sterile cell cultures, but ultimately cause alterations in cell characteristics and processes, leading to inaccurate experimental conclusions.

    We also offer:

    • De-Plasma™ II (D030)
    • PCR Mycoplasma Detection Kit (M034)
    Microbiology Applications De-Plasma™ II is a safe, effective anti-Mycoplasma reagent to avoid or eliminate Mycoplasma contamination. Use at 5 μg/ml for primary cells in culture and 2.5 μg/ml for prophylactic use.
    Eukaryotic Cell Culture Applications De-Plasma™ (TOKU-E) was used to routinely test carcinoma cell lines (HNSCC cell line) including SNU1076, FADU, HSC-2 and HSC-4 (Bdarny M et al, 2018). De-Plasma was also used to authenticate the human papilloma virus negative cells (Elkabets et al, 2015).
    References

    Drexler, HG (2002)  Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnol. 39(2):75-90

    Van Kuppeveld, FJM (1994)  Detection of Mycoplasma contamination in cell cultures by a Mycoplasma group-specific PCR.  Appl. Environ. Microbiol. 60(1): 149-152

    References for De-Plasma™ (TOKU-E)

    Bdarny M et al (2018)  The AP-1 complex regulates AXL expression and determines sensitivity to P13Ka inhibition in esophagus and head and neck squamous cell carcinoma. b

    Elkabets M et al (2015)  AXL mediates resistance to PI3K alpha inhibition by activating the EGFR/PKC/mTOR axis in head and neck and esophageal squamous cell carcinomas. Cancer Cell. 27(4):533-546  PMID 25873175