Dihydrostreptomycin Sulfate, a derivative of streptomycin, is a semi-synthetic aminoglycoside with bactericidal properties. The antibiotic interferes with bacterial protein synthesis and is commonly used to study aminoglycoside uptake. It is freely soluble in aqueous solution.
|Mechanism of Action||Aminoglycosides target the 30S ribosomal subunit (irreversible binding to S12 protein) resulting in an inability to read mRNA since this interferes with the initiation complex between the mRNA and the bacterial ribosome. This leads to the synthesis of faulty proteins which results in death of the bacterial cell. |
Dihydrostreptomycin inhibited the induction of beta-galactosidase in E. coli. It also caused accumulation of pyruvate, similar to known inhibitors of terminal respiration (Bragg and Polglase 1963).
|Spectrum||Activity against most Gram-positive and Gram-negative organisms, and Mycobacterium.|
|Microbiology Applications|| |
Dihydrostreptomycin can be used as a selective agent in several types of isolation media:
STAA Agar - STAA Selective Supplement
STAA Agar - STA Selective Supplement
|Eukaryotic Cell Culture Applications||Research on the use of SPLVs (stable plurilamellar vesicles) to entrap aminoglycosides as a drug carrier. Murine peritoneal macrophases and guinea pig peritoneal macrophages were used to study infections caused by Brucella species (Brucella canis and Brucella abortus) with effective elimination of intracellular bacteria (Fountain et al, 1985). |
Mouse fibroblasts (LM-strain (CCL1.2)) were used in a sensitive, reliable cell culture assay to determine the water quality of oil-refinery effluents, and Dihydrostreptomycin Sulfate was incorporated in the media to prevent contamination (Richardson, 1977).
|Molecular Formula|| |
(C21H41N7O12)2 •3 H2SO4
|References||Bragg PD and Polglase WJ (1963) Action of Dihydrostreptomycin and antagonism by cations. J. Bacteriol. 85(3): 590-94 PMID 14042936 |
Fountain MW, Weiss SJ, Fountain AG, Shen A, Lenk RP (1985) Treatment of Brucella canis and Brucella abortus in vitro and in vivo by stable plurilamellar vesicle-encapsulated aminoglycosides. J. Infect. Dis 152(3):529-535 PMID 2411828
Richardson, D et al (1977) Evaluation of a cell culture assay for determination of water quality of oil-refinery. Environ. Contam. Toxicol. (1977) 18: 683-690 PMID 597623