Hydrocortisone, USP (syn: Cortisol) is a glucocorticoid steroid hormone produced in the adrenal cortex. It can be used as a supplement in culture medium for oligodendrocytes, colon cells, and epithelial cells.
Hydrocortisone, USP is soluble in chloroform:absolute ethanol (1:1), sparingly soluble in ethanol and propylene glycol, slightly soluble in methanol and acetone, and practically insoluble in water.
| Mechanism of Action | Hydrocortisone interacts with glucocorticoid receptors and regulates various signaling pathways. Hydrocortisone inhibits the bioactivity of the proinflammatory cytokine interleukin 6 (Briegal et al, 2001). |
| Eukaryotic Cell Culture Applications |
Hydrocortisone has been used as a supplement in culture media to enhance proliferation and differentiation of oligodendrocyte, colon, epithelial, and mesenchymal cells. |
| References |
Briegel J, Jochum M, Gippner-Steppert C and Thiel M (2001) Immunomodulation in septic shock: Hydrocortisone differentially regulates cytokine responses. J. Am. Soc. Nephrol 12 (suppl 1) S70-S74 Fonseca MO, Godoi H, Da Silva NS and Nacheco-Soares C (2022) Evaluation of the effect of Hydrocortisone in 2D and 3D HEp-2 cell culture. In: Bastos-Filho, T.F., de Oliveira Caldeira, EM Frizera-Neto, A. (eds) XXVII Brazilian Congress on Biomedical Engineering. CBEB 2020. IFMBE Proc. vol 83. Springer, Cham Goodman RS, Johnson DB, Balko JM (2023) Corticosteroids and cancer immunotherapy. Clin Cancer Res. 14;29(14):2580-2587 PMID 36648402 |
