SKU: I011  / 
    CAS Number: 367-93-1

    IPTG (Isopropyl β-D-1-thiogalactopyranoside)

    ₩92,944.50 - ₩419,331.00

    IPTG (syn: Isopropyl β-D-1-thiogalactopyranoside) is a compound that induces transcription of the lac operon by  mimicking allolactose, a lactose metabolite. IPTG is routinely used as a tool in cloning and gene recombination to confirm successful gene insertion. It is used with X-Gal or Gluo-Gal in chromogenic substrate in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli.  

    IPTG is soluble in water.

    Mechanism of Action IPTG is an inducer of the lactose system.  It induces the lac operon by binding to and releasing the lac repressor allowing transcription of lac genes including beta-galactosidase.
    Microbiology Applications

    IPTG can be used to confirm successful DNA recombination within the lac operon in cloning experiments. X-gal, or Bluo-Gal chromogenic substrate which turns a blue color when cleaved by IPTG can be used in the media in which the cells are grown. White colonies indicate a gene insertion within the lac operon because beta-galactosidase was not expressed. Blue colonies are indicative of an intact lac operon which expressed beta-galactosidase and subsequently cleaved X-gal.

    References

    Hansen L, Knudsen S and Sørensen S (1998) The effect of the lacY gene on the induction of IPTG inducible promoters, studied in Escherichia coli and Pseudomonas fluorescens.  Curr Microbiol 36:341–347 

    Lewis M et al (1996)  Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Science 271(5253):1247-254

    Malakar P and Venkatesh KV (2012)  Effect of substrate and IPTG concentrations on the burden to growth of Escherichia coli on glycerol due to the expression of Lac proteins. Appl Microbiol Biotechnol 93:2543–2549