Pencillin-Streptomycin (Pen/Strep) is an antibacterial solution comprised of penicillin (10,000 units/mL), a beta-lactam, and streptomycin (10,000 ug/mL), an aminoglycoside in 0.9% NaCl.
Dr. Chung et al. used penicillin-streptomycin from TOKU-E in their RPMI 1640 medium to culture HT-29 colon cancer cells. Read more here: "Rab11 collaborates E-cadherin to promote collective cell migration and indicates a poor prognosis in colorectal carcinoma"
|Mechanism of Action||Penicillin, the beta lactam, breaks peptidoglycan links in non-resistant cell walls and triggers autolysins which kill the cell. Streptomycin, the aminoglycoside, binds to the ribosomes and inhibits protein synthesis.|
|Spectrum||Penicillin-streptomycin solution targets the cell wall of both Gram-positive and Gram-negative bacteria.|
|Eukaryotic Cell Culture Applications||Penicillin-streptomycin solution is routinely used in eukaryotic cell culture applications to prevent or control contamination caused by Gram-positive or Gram-negative bacteria. Penicillin-streptomycin solution should be used at a concentration of 50-100 Units/mL penicillin and 50-100 µg/mL streptomycin.penicillin in cell culture media.|
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