SKU: S016  / 
    CAS Number: 64058-48-6

    Spectinomycin sulfate

    €23,85 - €95,15

    Spectinomycin Sulfate is an aminocyclitol antibiotic that is isolated from Streptomyces spectabilis.  Spectinomycin was discovered in culture broth by researchers at the Upjohn Company in 1961. Spectinomycin Sulfate shows activity against gram-positive bacteria, gram-negative bacteria, and Mycoplasma. 

    Spectinomycin Sulfate is a protein synthesis inhibitor.  It acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation. This eventually leads to bacterial cell death.

    Spectinomycin is commonly used as a selective agent to isolate cells that contain aadA spectinomycin resistance genes.  It also has various uses in plant biology applications.

    TOKU-E offers two forms of spectinomycin: spectinomycin DiHCl (S006) and Spectinomycin sulfate (S016). In aqueous solution, spectinomycin DiHCl is freely soluble, and spectinomycin sulfate is sparingly soluble (0.150 mg/mL).

    Synonyms: Togamycin, Trobicin, Spectam, Actinospectacin, U-18409E

    Mechanism of ActionThe aminocyclitol antibiotic spectinomycin, often considered alongside the aminoglycosides, binds in reversible fashion (hence the bacteriostatic activity) to the 16S rRNA of the ribosomal 30S subunit. There it interrupts the translocation event that occurs as the next codon of mRNA is aligned with the A site in readiness for the incoming aminoacyl-tRNA. Structural studies reveal that the antibiotic binds to an area of the 30S subunit known as the head region which needs to move during translocation. Binding of the rigid spectinomycin molecule appears to prevent the movement required for translocation.
    SpectrumSpectinomycin sulfate is an antibiotic that is active against a variety of aerobic gram-negative and gram-positive organisms as well as Mycoplasma species. Spectinomycin is used clinically, primarily for its activity against gram-negative organisms; some gram-positive organisms may also be susceptible to this agent.  Anaerobic organisms are generally resistant. Spectinomycin is usually bacteriostatic at therapeutic doses.
    Impurity Profile

    Related Substances:
    Impurity A,E (each):  Not more than 1.0%
    Impurity D: Not more than 4.0%
    Any other impurity: Not more than 1.0%
    Total impurities: Not more than 6.0%

    Microbiology ApplicationsSpectinomycin sulfate is commonly as a selective agent to isolate cells that contain aadA spectinomycin resistance genes. Spectinomycin sulfate is typically used at concentrations between 50 - 100 µg/mL.
    Plant Biology ApplicationsSpectinomycin sulfate has been use to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media
    Eukaryotic Cell Culture ApplicationsSpectinomycin sulfate is suitable for cell culture applications. Recommended concentration for cell culture use is at 7.5-20 mg/L.
    Molecular FormulaC14H26N2O11S•H2SO4•4H2O
    References

    Thompson, P.R., et al. 1998. J. Biol. Chem. 273: 14788-14795. PMID: 9614079 

    Zimmerman, J.M., et al. 2002. Nucleic Acids Res. 30: 5425-5435. PMID: 12490711 

    Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50. 

    Thom, G. and Prescott, C.D. 1997. Bioorg. Med. Chem. 5: 1081-1086. PMID: 9222501 


    Ian Chopra, CHAPTER 2 - Modes of action, Antibiotic and Chemotherapy (Ninth Edition), W.B. Saunders, 2010, Pages 10-23, ISBN 9780702040641

    Anderson, P. 1969. J. Bacteriol. 100: 939-947. PMID: 16559073 

    Suter, T.M., et al. 1997. Antimicrob. Agents Chemother. 41: 1385-1388. PMID: 9174205

    MICBorrelia afzelii | 0.25 - 1|| Borrelia bissettii | 2|| Borrelia burgdorferi| 0.25 - 2|| Borrelia garinii| 0.25 - 0.5|| Borrelia valaisiana| 2|| Chlamydia psittaci| ≥0.3 - 10000|| Chlamydia trachomatis| ≥0.8|| Diplococcus pneumoniae| 6.3 - 25|| Escherichia coli| 0.5 - >512|| Haemophilus influenzae| 3.1 - 12.5|| Haemophilus parasuis| 4 - 128|| Lactobacillus reuteri| 2500|| Mycoplasma bovis | 0.12 - 8|| Mycoplasma gallisepticum| 0.39 - 1|| Mycoplasma hyopneumoniae| ≤0.12 - 2|| Mycoplasma hyosynoviae| ≥4|| Mycoplasma synoviae | 0.39 - 6.25|| Neisseria gonorrhoeae | 1 - 32|| Neisseria spp.| 4 - 64|| Nocardia asteroides| 6.3 - ≥800|| Pasteurella haemolytica| ≥32|| Pseudomonas aeruginosa| 32 - 256|| Salmonella typhimurium (LT2)| ≥32||