Ampicillin Sodium ReadyMadeTM Aqueous Solution is provided as a sterile-filtered solution of Ampicillin Sodium formulated in water at a concentration of 100 mg/ml. It has been filter-sterilized using a 0.22 μm filter.
Ampicillin Sodium is a member of the β-lactam family structurally similar to penicillin. It has an amino group side chain attached to the penicillin structure, 6-amino-penicillanic acid. Ampicillin resistance is used as a selectable marker to confirm successful cell transformation, as only cells containing plasmid-encoded ESBLs (Extended Spectrum B-lactamases) survive.
We also offer:
- Ampicillin Sodium (A042)
Mechanism of Action | Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBPs are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis. |
Spectrum | Ampicillin is broad-spectrum, effective against both Gram-positive and Gram-negative bacteria including Gram-negative non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, Ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to Ampicillin is routinely used as a selectable marker to confirm successful cell transformation. |
Microbiology Applications |
Ampicillin Sodium is often used to select for cells that have been transformed with a plasmid containing the bla gene which confers resistance to Ampicillin. Ampicillin Sodium is typically used at a concentration of 50-100 µg/mL. It can also be used with pBR322 and pUC derivatives. Media Supplements Aeromonas Medium Base - Ampicillin Selective Supplement |
Eukaryotic Cell Culture Applications | Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information, please visit our Cell-Culture Database. |
Molecular Formula | C16H18N3NaO4S |
References |
Cure E, Kucuk A and Cure MC (2020) Cyclosporine therapy in cytokine storm due to coronavirus disease 2019 (COVID-19). Rheumatol. Int. 40:1177-1179 Homan KA et al (2016) Bioprinting of 3D convoluted renal proximal tubules on perfusable chips. Sci Rep. 11(6):34845 PMID 27725720 Laupacis A et al (1982) Cyclosporin A: A powerful immunosuppressant. Can. Med Assoc. J 126(9):1041-1046 PMID 7074504 Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in Gram-negative bacilli. Am. J. Med 103(1):51-59 PMID 9236486 Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem 52:825-869 PMID 6351730 Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coli using two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011 Zhang G and Lyons JG (2002) Cyclosporin A improves the selection of cells transfected with the Puromycin acetyltransferase gene. Biotechniques, 33(1):32-36 |