Cefotaxime ReadyMadeTM Solution is a sterile-filtered solution of Cefotaxime Sodium, USP at a concentration of 100 mg/ml that has been dissolved in water and filter sterilized (0.2 μm) ready for use in your application. Cefotaxime Sodium is a broad-spectrum, third-generation cephalosporin and a penicillin derivative.
We also offer:
- Cefotaxime Sodium, USP (C011)
|Mechanism of Action||
Cephalosporins are β-lactam antibiotics and interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBPs are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Cefotaxime Sodium however, is largely resistant to β-lactamases.
|Spectrum||Cefotaxime Sodium has broad-spectrum activity against a wide variety of Gram-positive and Gram-negative bacteria. However, unlike many other cephalosporin antibiotics, Cefotaxime Sodium is not effective against Pseudomonas aeruginosa.|
Cefotaxime Sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
Neisseria gonorrhoeae 0.008 µg/mL – 0.03 µg/mL
|Plant Biology Applications||
Cefotaxime is often used in Agrobacterium tumefaciens mediated transformations to combat bacterial growth. Typical working concentration for this application is 100-500 mg/L. It is sometimes used in combination with Vancomycin as it has a synergistic effect.
Ferrer-González E, Kaul M, Parhi AK, LaVoie EJ and Pilch DS (2017) Lactam antibiotics with a high affinity for PBP2 act synergistically with the FtsZ-targeting agent TXA707 against methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 61(9):e00863-17 PMID 28630190
Georgopapadakou NH (1992) Mechanisms of action of cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli. Antimicrob. Agents. Chemother. 37(3): 559-65 PMID 8384817
Lefurgy ST et al (2016) Analysis of the structure and function of FOX-4 cephamycinase. Antimicrob. Agents. Chemother. 60 (2):717-728 PMID 26525784
Mathias RJ and Boyd LA (1986) Cefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat (Triticum aestivum L. em Thell). Plant Sci. 46:217-223
Shackelford NJ, and Chlan C (1996) Identification of antibiotics that are effective in eliminating 2 Agrobacterium tumefaciens. Plant Molecular Biology Reporter 14(1):50-57 Merck 13, 1946
Fabr H, Eddine NH, Berge EG (1984) Degradation kinetics in aqueous solution of Cefotaxime Sodium, a third-generation cephalosporin,