SKU: C293-10X1ML  / 
    CAS Number: 64485-93-4

    Cefotaxime ReadyMade™ Solution


    Cefotaxime ReadyMadeTM Solution is a sterile-filtered solution of Cefotaxime Sodium, USP at a concentration of 100 mg/ml that has been dissolved in water and filter sterilized (0.2 μm) ready for use in your application. Cefotaxime Sodium is a broad-spectrum, third-generation cephalosporin and a penicillin derivative.

    We also offer:

    • Cefotaxime Sodium, USP (C011)

    Mechanism of Action

    Cephalosporins are β-lactam antibiotics and interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBPs are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Cefotaxime Sodium however, is largely resistant to β-lactamases.

    Spectrum Cefotaxime Sodium has broad-spectrum activity against a wide variety of Gram-positive and Gram-negative bacteria. However, unlike many other cephalosporin antibiotics, Cefotaxime Sodium is not effective against Pseudomonas aeruginosa.
    Microbiology Applications

    Cefotaxime Sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:

    Neisseria gonorrhoeae 0.008 µg/mL – 0.03 µg/mL
    Streptococcus pneumoniae ≤0.007 µg/mL - 0.25 µg/mL
    For a representative list of Cefotaxime MIC values, click here.

    Plant Biology Applications

    Cefotaxime is often used in Agrobacterium tumefaciens mediated transformations to combat bacterial growth. Typical working concentration for this application is 100-500 mg/L. It is sometimes used in combination with Vancomycin as it has a synergistic effect.

    Molecular Formula C16H16N5NaO7S2 

    Ferrer-González E, Kaul M, Parhi AK, LaVoie EJ and Pilch DS (2017) Lactam antibiotics with a high affinity for PBP2 act synergistically with the FtsZ-targeting agent TXA707 against methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 61(9):e00863-17 PMID 28630190

    Georgopapadakou NH (1992) Mechanisms of action of cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli. Antimicrob. Agents. Chemother. 37(3): 559-65 PMID 8384817

    Lefurgy ST et al (2016) Analysis of the structure and function of FOX-4 cephamycinase. Antimicrob. Agents. Chemother. 60 (2):717-728 PMID 26525784

    Mathias RJ and Boyd LA (1986) Cefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat (Triticum aestivum L. em Thell). Plant Sci. 46:217-223

    Shackelford NJ, and Chlan C (1996) Identification of antibiotics that are effective in eliminating 2 Agrobacterium tumefaciens. Plant Molecular Biology Reporter 14(1):50-57 Merck 13, 1946

    Fabr H, Eddine NH, Berge EG (1984) Degradation kinetics in aqueous solution of Cefotaxime Sodium, a third-generation cephalosporin,
    J. Pharm. Sci. 73 (5): 611-618