Ampicillin Sodium ReadyMadeTM Aqueous Solution is provided as a sterile-filtered solution of Ampicillin Sodium formulated in water at a concentration of 100 mg/ml. It has been filter-sterilized using a 0.22 μm filter.
Ampicillin Sodium is a member of the β-lactam family structurally similar to penicillin. It has an amino group side chain attached to the penicillin structure, 6-amino-penicillanic acid. Ampicillin resistance is used as a selectable marker to confirm successful cell transformation, as only cells containing plasmid-encoded ESBLs (Extended Spectrum B-lactamases) survive.
We also offer:
- Ampicillin Sodium (A042)
| Mechanism of Action | Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBPs are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis. |
| Spectrum | Ampicillin is broad-spectrum, effective against both Gram-positive and Gram-negative bacteria including Gram-negative non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, Ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to Ampicillin is routinely used as a selectable marker to confirm successful cell transformation. |
| Microbiology Applications |
Ampicillin Sodium is often used to select for cells that have been transformed with a plasmid containing the bla gene which confers resistance to Ampicillin. Ampicillin Sodium is typically used at a concentration of 50-100 µg/mL. It can also be used with pBR322 and pUC derivatives. Media Supplements Aeromonas Medium Base - Ampicillin Selective Supplement |
| Eukaryotic Cell Culture Applications | Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information, please visit our Cell-Culture Database. |
| Molecular Formula | C16H18N3NaO4S |
| References |
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