Anhydrotetracycline hydrochloride (ATC) is a tetracycline derivative routinely used in tetracycline mediated gene expression. Anhydrotetracycline hydrochloride does not have antibiotic properties and is soluble in ethanol (2 mg/mL) and DMSO (10 mg/mL).
|Eukaryotic Cell Culture Applications||Anhydrotetracycline acts as an inducer of tetracycline resistance genes by binding to and inactivating the tetracycline repressor TetR to allow expression of downstream genes of the tetr promoter. Tetracycline also acts as a inducer of tetracycline resistance genes; however, anhydrotetracycline binds to the TetR repressor with higher affinity without toxic effects.|
|Molecular Formula||C22H22N2O7 · xHCl (lot specific)|
|References||Gossen, Manfred. "Anhydrotetracycline, a Novel Effector for Tetra." Nucleic Acids Research 21.18 (1993): 4411-412. Ncbi.gov. Web. 3 May 2013. Skerra, Arne. "Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in< i> Escherichia coli." Gene 151.1 (1994): 131-135.|