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Detailed Description
CAS Number: 42540-40-9
Molecular Formula: C19H17N6NaO6S2
Molecular Weight: 512.49
Mechanism of Action: Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases.
Storage Conditions: -20°C
Tariff Code: 2941.90.3000
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Applications
Spectrum: Cefamandole is a broad-spectrum cephalosporin targeting a wide variety of Gram-positive and Gram- negative bacteria.
Microbiology Applications:
Cefamandole Nafate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.
Representative MIC values include:
- Escherichia coli 0.08 µg/mL – 100 µg/mL
- Klebsiella pneumoniae 0.8 µg/mL – 12.5 µg/mL
- For a complete list of Cefamandole MIC values, click here.
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Specifications
Form: Powder
Appearance: White or almost white powder
Source: Semi-synthetic
Water Content (Karl Fischer): ≤2.0%
pH: 3.5-7.0
Assay: (On Dried Basis): 810-1000µg/mg
Identification: HPLC, IR, UV, Flame reaction of Na
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Technical Data
Solubility: Methanol: Sparingly soluble
Water: SolubleImpurity Profile: |Cefamandole nafate|42540-40-9|C19H17N6NaO6S2|512.5| |Cefamandole sodium|30034-03-8|C18H17NaO5S2|484.5| Impurity A| formylmandeloyl-7-amino-desacetoxy-cephalosporanic acid||C16H16N2O6S|364.4| Impurity C| O-acetylcefamandole||C18H20N6O6S2|480.5| Impurity D| 1-methyl-1H-tetrazole-5-thiol|69713-31-1|C2H4N4S|16.145| Impurity E| formylmandeloyl-7-ACA||C19H18N2O8S|434.4|
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References
References:
Georgopapadakou NH (1992) Mechanisms of action of Cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli. Antimicrob. Agents Chemother. 37(3):559-565.
Gupta D, V, Stewart KR (1981) Stability of Cefamandole Nafate and cefoxitin sodium solutions. Am. J. Hosp. Pharm. 38(6):875-879 PMID 7246563
Cefamandole Nafate, USP is a second-generation cephalosporin and beta-lactam antibiotic with bactericidal activity. It is freely soluble in aqueous solution and sparingly soluble in methanol. The ester compound, Nefamandole Nafate is hydrolyzed to Cefamandole.
Cefamandole has been used to study the expression of penicillin-binding proteins on bacterial cell walls and antibiotic resistance mechanisms.
We also offer:
- Cefamandole Sodium (C079)
CAS Number: 42540-40-9
Molecular Formula: C19H17N6NaO6S2
Molecular Weight: 512.49
Mechanism of Action: Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases.
Storage Conditions: -20°C
Tariff Code: 2941.90.3000
Spectrum: Cefamandole is a broad-spectrum cephalosporin targeting a wide variety of Gram-positive and Gram- negative bacteria.
Microbiology Applications:
Cefamandole Nafate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.
Representative MIC values include:
- Escherichia coli 0.08 µg/mL – 100 µg/mL
- Klebsiella pneumoniae 0.8 µg/mL – 12.5 µg/mL
- For a complete list of Cefamandole MIC values, click here.
Form: Powder
Appearance: White or almost white powder
Source: Semi-synthetic
Water Content (Karl Fischer): ≤2.0%
pH: 3.5-7.0
Assay: (On Dried Basis): 810-1000µg/mg
Identification: HPLC, IR, UV, Flame reaction of Na
Solubility: Methanol: Sparingly soluble
Water: Soluble
Impurity Profile: |Cefamandole nafate|42540-40-9|C19H17N6NaO6S2|512.5| |Cefamandole sodium|30034-03-8|C18H17NaO5S2|484.5| Impurity A| formylmandeloyl-7-amino-desacetoxy-cephalosporanic acid||C16H16N2O6S|364.4| Impurity C| O-acetylcefamandole||C18H20N6O6S2|480.5| Impurity D| 1-methyl-1H-tetrazole-5-thiol|69713-31-1|C2H4N4S|16.145| Impurity E| formylmandeloyl-7-ACA||C19H18N2O8S|434.4|
References:
Georgopapadakou NH (1992) Mechanisms of action of Cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli. Antimicrob. Agents Chemother. 37(3):559-565.
Gupta D, V, Stewart KR (1981) Stability of Cefamandole Nafate and cefoxitin sodium solutions. Am. J. Hosp. Pharm. 38(6):875-879 PMID 7246563
