Cefoperazone Free Acid is a semi-synthetic, broad-spectrum third-generation cephalosporin. Patented in 1974, it was marketed by Pfizer under the trade name Cefobid. The compound has activity against Pseudomonas. It has a tetrazolyl moiety that confers β-lactamase resistance. Cefoperazone may be used as an analytical standard for the determination of cefoperazone in raw bovine milk.
Cefoperazone Free Acid is slightly soluble in DMSO and Methanol.
| Mechanism of Action | Cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. |
| Spectrum | Cefoperazone Free Acid has broad-spectrum activity, including activity against Pseudomonas. It is effective against a wide variety of Gram-positive and Gram-negative bacteria. |
| Microbiology Applications | Cefoperazone is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.
Representative MIC values include: Haemophilus influenzae: 0.12 - 0.25 µg/ml For a representative list of Cefoperazone MIC values, click here. |
| Molecular Formula | C25H27N9O8S2 |
| References |
Crosby MA and Gump DW (1982) Activity of Cefoperazone and two beta-lactamase inhibitors, sulbactam and clavulanic acid, against Bacteroides spp. correlated with beta-lactamase production. Antimicrob. Agents Chemother. 22(3):398-405 PMID 6291451 Matsubara N, Minami S, Muraoka T, Saikawa I and Mitsuhashi S (1979) In vitro antibacterial activity of Cefoperazone (T-1551), a new semisynthetic cephalosporin. Antimicrob. Agents Chemother. 16(6):731-735 PMID 316988 |
