SKU: D108-10x0.5 ML  / 
    CAS Number: 103060-53-3

    Daptomycin ReadyMade™ Solution

    NT$12,306.16
    Daptomycin ReadyMade™ Solution
    • Detailed Description

      CAS Number: 103060-53-3

      Molecular Formula: C72H101N17O26

      Molecular Weight: 1620.67

      Mechanism of Action: Daptomycin peptides interact with anionic phospholipids in the membrane of Gram-positive bacteria. Peptides integrate into the membrane by a calcium-mediated process and interact with membrane phospholipid head groups and acyl chains. Finally, a pore or channel is formed which can kill the cell by membrane disruption/depolarization or cellular uptake of Daptomycin leading to disruption of intracellular processes such as synthesis of DNA, RNA, and protein which results in bacterial cell death. The activity of Daptomycin is dependent on the presence of physiologic levels of free calcium ions. Researchers found a gradual dissipation of membrane potential, thus membrane depolarization is an inherent part of its mechanism of action (Silverman et al, 2003).

      Storage Conditions: -20°C

      Tariff Code: 2941.90.3000

    • Applications

      Spectrum: Daptomycin targets Gram-positive organisms including GRE (glycopeptide-resistant Enterococci) and MRSA (methicillin-resistant Staphylococcus aureus).

      Microbiology Applications:

      Daptomycin has been used to study the impact of Staphylococcal accessory regulator (sarA) on Daptomycin susceptibility of Staphylococcus aureus. The mutation of sarA did not affect the Daptomycin MIC but resulted in increased susceptibility in vivo in the context of an established biofilm (Weiss et al, 2009).

      Representative MIC ranges for Daptomycin from our Antimicrobial Index appear below.

      Vancomycin-resistant Enterococcus faecalis - ≤0.015 μg/mL － 4 μg/mL
      MRSA: 0.25 μg/mL - 2 μg/mL

    • Specifications

      Form: Solution

      Source: Streptomyces roseosporus

    • References

      References:

      Baltz RH, Miao V and Wrigley SK (2005) Natural products to drugs: Daptomycin and related lipopeptide antibiotics. Nat. Prod. Rep. 22(6):717-741 PMID 16311632

      McHenney MA Hosted TJ, Dehoff BS, Rosteck PR and Baltz RH (1998) Molecular cloning and physical mapping of the Daptomycin gene cluster from Streptomyces roseosporus. J. Bacteriol. 180(1):143-151

      Miao V et al (2006) Genetic engineering in Streptomyces roseosporus to produce hybrid lipopeptide antibiotics. Chem. Biol. 13(3):269-76 PMID 16638532

      Rotondi KS and Gierasch LM (2005) A well-defined amphipathic conformation for the calcium-free cyclic lipopeptide antibiotic, Daptomycin, in aqueous solution. Peptide Sci. 80(2-3):374-385

      Silverman JA, Perlmutter NG, and Shapiro HM (2003) Correlation of Daptomycin bactericidal activity and membrane depolarization in Staphylococcus aureus. Antimicrob. Agents Chemother. 47(8):2538-44 PMID 12878516

      Straus, SK and Hancock REW (2006) Mode of action of the new antibiotic for Gram-positive pathogens Daptomycin: Comparison with cationic antimicrobial peptides and lipopeptides. Biomem. 1758(9):1215-1223

      Taylor SD and Palmer M (2016) The action mechanism of Daptomycin. Bioorganic and Med. Chem. 24(24):6253-6268

      Weiss EC et al (2009) Impact of sarA on Daptomycin susceptibility of Staphylococcus aureus biofilms in vivo. Antimicrobial. Agents. Chemother. 53(10):4096-4102 PMID 19651914

    Daptomycin ReadyMade™ Solution is provided as a sterile-filtered solution formulated in ~ 10% β-cyclodextrin saline solution at a concentation of  1 mg/ml.

    Daptomycin is a unique 13-residue cyclic anionic lipopeptide antibiotic derived from Streptomyces roseosporus that is effective against Gram-positive bacteria. This holoenzyme is composed of three subunits, encoded by the dptA, dptBC, and dptD genes, each responsible for incorporating particular amino acids into the peptide. The cloned dpt gene cluster allows study of Daptomycin biosynthesis and discovery of novel lipopetides through pathway engineering.

    Resarch has shown that Daptomycin requires calcium to form a micellar structure in solution and to bind to bacterial model membranes.

    We also offer:

    CAS Number: 103060-53-3

    Molecular Formula: C72H101N17O26

    Molecular Weight: 1620.67

    Mechanism of Action: Daptomycin peptides interact with anionic phospholipids in the membrane of Gram-positive bacteria. Peptides integrate into the membrane by a calcium-mediated process and interact with membrane phospholipid head groups and acyl chains. Finally, a pore or channel is formed which can kill the cell by membrane disruption/depolarization or cellular uptake of Daptomycin leading to disruption of intracellular processes such as synthesis of DNA, RNA, and protein which results in bacterial cell death. The activity of Daptomycin is dependent on the presence of physiologic levels of free calcium ions. Researchers found a gradual dissipation of membrane potential, thus membrane depolarization is an inherent part of its mechanism of action (Silverman et al, 2003).

    Storage Conditions: -20°C

    Tariff Code: 2941.90.3000

    Spectrum: Daptomycin targets Gram-positive organisms including GRE (glycopeptide-resistant Enterococci) and MRSA (methicillin-resistant Staphylococcus aureus).

    Microbiology Applications:

    Daptomycin has been used to study the impact of Staphylococcal accessory regulator (sarA) on Daptomycin susceptibility of Staphylococcus aureus. The mutation of sarA did not affect the Daptomycin MIC but resulted in increased susceptibility in vivo in the context of an established biofilm (Weiss et al, 2009).

    Representative MIC ranges for Daptomycin from our Antimicrobial Index appear below.

    Vancomycin-resistant Enterococcus faecalis - ≤0.015 μg/mL － 4 μg/mL
    MRSA: 0.25 μg/mL - 2 μg/mL

    Form: Solution

    Source: Streptomyces roseosporus

    References:

    Baltz RH, Miao V and Wrigley SK (2005) Natural products to drugs: Daptomycin and related lipopeptide antibiotics. Nat. Prod. Rep. 22(6):717-741 PMID 16311632

    McHenney MA Hosted TJ, Dehoff BS, Rosteck PR and Baltz RH (1998) Molecular cloning and physical mapping of the Daptomycin gene cluster from Streptomyces roseosporus. J. Bacteriol. 180(1):143-151

    Miao V et al (2006) Genetic engineering in Streptomyces roseosporus to produce hybrid lipopeptide antibiotics. Chem. Biol. 13(3):269-76 PMID 16638532

    Rotondi KS and Gierasch LM (2005) A well-defined amphipathic conformation for the calcium-free cyclic lipopeptide antibiotic, Daptomycin, in aqueous solution. Peptide Sci. 80(2-3):374-385

    Silverman JA, Perlmutter NG, and Shapiro HM (2003) Correlation of Daptomycin bactericidal activity and membrane depolarization in Staphylococcus aureus. Antimicrob. Agents Chemother. 47(8):2538-44 PMID 12878516

    Straus, SK and Hancock REW (2006) Mode of action of the new antibiotic for Gram-positive pathogens Daptomycin: Comparison with cationic antimicrobial peptides and lipopeptides. Biomem. 1758(9):1215-1223

    Taylor SD and Palmer M (2016) The action mechanism of Daptomycin. Bioorganic and Med. Chem. 24(24):6253-6268

    Weiss EC et al (2009) Impact of sarA on Daptomycin susceptibility of Staphylococcus aureus biofilms in vivo. Antimicrobial. Agents. Chemother. 53(10):4096-4102 PMID 19651914