In the literature

TOKU-E Company products are widely distributed to major life science companies and research labs around the world. Over the past several years, scientists in various disciplines from cell culture to chemical engineering have trusted the quality of TOKU-E’s products for use in their research. Our reputation for quality products has led to numerous publications citing our products. Below, we have summarized some of the interesting research our clients have done.

If you are starting a research project, our product catalog is a great resource to help you on your journey to discovery.

• Analytical Chemistry

  Poller et al. 
  used Enrofloxacin HCl from TOKU-E to study different methods for detecting enrofloxacin in milk.
  Read more here: "Influence of different surface chemistries on the ultrasensitive on-chip detection of enrofloxacin in milk"

  Waterman et al. 
  used Bacitracin F, EvoPure from TOKU-E as a reference standard to confirm the formation of the degradant product, bacitracin F from the active, bacitracin A.
  Read more here: "Accelerated Stability Modeling for Peptides: a Case Study with Bacitracin."
  
  Lim et al. 
  used Polymyxin B1, B2, B3, and B1-I from TOKU-E to study the stability of each compound in saline, dextrose, and saline/dextrose infusion solutions.
  Read more here: "Physicochemical stability study of polymyxin B in various infusion solutions for administration to critically Ill patients." 

• Biochemistry

  Huang et al. 
  used Kanamycin B Sulfate (TOKU-E) to study enzymatic conversion of gentamicin A2 to other gentamicin congeners.
  Read more here: "Delineating the Biosynthesis of Gentamicin X2, the Common Precursor of the Gentamicin C Antibiotic Complex."  

  Guo et al. 
  used Gentamicin X2 sulfate, EvoPure from TOKU-E to study its conversion to G418 by C-methylation at C-6'.
  Read more here: "Specificity and Promiscuity at the Branch Point in Gentamicin Biosynthesis." 

• Mammalian Cell Culture

  Lu et al. 
  used Blasticidin S HCl and Puromycin Diihydrochloride from TOKU-E to select for transfected AS-B145 and BT-474 cells. 
  Read more here: "Ovatodiolide Inhibits Breast Cancer Stem/Progenitor Cells through SMURF2-Mediated Downregulation of Hsp27"
  
  Conti et al. 
  used Puromycin DiHCl from TOKU-E to select for eGFP expressing A549 cells.
  Read more here: "Polymeric Nanocarriers And Their Oral Inhalation Forumlations For The Regional Delivery of Nucleic Acids To The Lungs."

  Sandoval-Jaime et al. 
  used Puromycin DiHCl from TOKU-E to select for stably transfected cells.
  Read more here: "Recovery of murine norovirus and feline calicivirus from plasmids encoding EMCV IRES in stable cell lines expressing T7 polymerase."

   

• Microbiology

Read more here:"Novel small molecules potentiate premature termination codon readthrough by aminoglycosides."

Caldwell et al. 

used Gentamicin C1 Sulfate from TOKU-E to study aminoglycoside binding sites of APH-GMPPNP and APH-GTP crystals.
Read more here: "Antibiotic Binding Drives Catalytic Activation of Aminoglycoside Kinase APH(2″)-Ia."  

Chen et al. 
used Rapamycin from TOKU-E to study the mammalian cellular target of rapamycin.
Read more here: "AMPA receptor–mTOR activation is required for the antidepressant-like effects of sarcosine during the forced swim test in rats: insertion of AMPA receptor may play a role" 

Choi et al. 
used Nitrocefin from TOKU-E to study nitrocefin hydrolysis activity of Ni-NTA proteins.
Read more here: "The interplay between effector binding and allostery in an engineered protein switch" 

Choi et al. 
measured enzymatic activity of engineered protein switches by exploiting Nitrocefin hydrolysis.
Read more here: "Electrochemical Activation of Engineered Protein Switches."

Dahlin et al. 
used Nitrocefin from TOKU-E in a surrogate ß-lactamase-nitrocefin assay.
Read more here: "A Cell-Free Fluorometric High-Throughput Screen for Inhibitors of Rtt109-Catalyzed Histone Acetylation."

Eze E et al. 
used Nitrocefin from TOKU-E to confirm beta-lactamase production in E. coli and Klebsiella species from Nigeria.
Read more here: "Occurrence of Beta-Lactamases and the Antibiogram Pattern of Clinical Isolates of Escherichia coli and Klebsiella Species in Nsukka Metropolis." 

Huang et al. 
used Nitrocefin from TOKU-E as a substrate to study the activity of VIM-2 Metallo-ß-lactamases (MBLs).
Read more here: "Inhibiting the VIM-2 Metallo-ß-Lactamase by Graphene Oxide and Carbon Nanotubes."

Kadir et al. 

used Cycloheximide from TOKU-E in culture medium to prevent de-novo translation of DVL proteins in neuroblastoma SH-SY5Y cells.
Read more here: "ALFY-Controlled DVL3 Autophagy Regulates Wnt Signaling, Determining Human Brain Size" 

Kiianitsa et al. 
used Nalidixic Acid from TOKU-E to study DNA gyrase inhibition in E. coli K-12.
Read more here: "Ultrasensitive isolation, identification and quantification of DNA–protein adducts by ELISA-based RADAR assay"

Langer et al. 
used Bacitracin UltraPure from TOKU-E to inhibit ATG-mediated TF activation and surface-associated PDI activity in HL60 cells.
Read more here: "Rapid Activiation of Monocyte Tissue Factor by Antithymocyte globulin is Dependent on Complement and protein Disulfide Isomerase."

Lefurgy et al. 
used Nitrocefin from TOKU-E to study cephamycin resistance in FOX enzymes which confer resistance to the cephamycin antibiotic, cefoxitin.
Read more here: "FOX-4 cephamycinase: an analysis of structure and function"

Liu et al. 
used Nitrocefin from TOKU-E to study and develop a homogeneous biosensor using a non-interfering blocking agent.
Read more here: "Parts-per-Million of Polyethylene Glycol as a Non-Interfering Blocking Agent for Homogeneous Biosensor Development."

Nicholes et al. 
used Nitrocefin from TOKU-E to study the enzymatic activity of protein switches.
Read more here: "Modular protein switches derived from antibody mimetic proteins" 

Ohlhoff et al. 

used Nitrocefin from TOKU-E as a substrate to study the activity of EstG34 ß-lactamases.
Read more here: "An unusual feruloyl esterase belonging to family VIII esterases and displaying a broad substrate range"

Pierre, et al. 
used Nitrocefin from TOKU-E to measure the enzymatic acivity of various beta-lactamases.
Read more here: "Molecular Determinants for Protein Stabilization by Insertional Fusion to a Thermophilic Host Protein."

Randall et al. 
used Nitrocefin from TOKU-E to study PenA B-lactamase activity - a beta-lactamase produced by Burkholderia pseudomallei which confers resistance to ceftazidime.
Read more here: "Membrane bound PenA B-lactamase of Burkholderia pseudomallei"

Tullman and Nicholes, et al. 
used Nitrocefin from TOKU-E to study and characterize enzymatic protein switches.
Read more here: "Enzymatic protein switches built from paralogous input domains."

Zhangming et al. 

used Nitrocefin from TOKU-E as a substrate to study TEM-1 ß-lactamase activity from E. coli.
Read more here: "Label-Free Measurements of Reaction Kinetics Using a Droplet-Based Optofluidic Device."  

• Plant Biology
• Hada et al. 
  used Carbenicillin from TOKU-E to select for resistant plants transformed with the thaumatin gene.
  Read more here: "Overexpression of thaumatin gene confers enhanced resistance to Alternaria brassicaeand tolerance to salinity and drought in transgenic Brassica juncea."  
• Virology
  Webb et al. 
  used Bacitracin F, EvoPure to study its effect on protein disulfide isomerase (PDI) inhibition.
  Read more here:"Dose-Response Models Reveal Critical Features of Inhibitor Activity and Viral Infection."