Neomycin ReadyMade™ Solution (10 mg/ml Neomycin sulfate in 0.9% NaCl) is a sterile, ready-to-use preparation of the broad-spectrum aminoglycoside Neomycin sulfate. The substance is composed of related compounds including Neomycin A (neamine), Neomycin B (framycetin), Neomycin C, and a few minor compounds. Neomycin B is the most active, followed by C and A. The quantities of these components vary from lot-to-lot.
We also offer:
- Neomycin Sulfate, USP (N003)
- Neomycin Sulfate, EP (N012)
- Neomycin B Sulfate, EvoPure® (N019)
- Neomycin C Sulfate, EvoPure® (N022)
Custom Manufacturing and Testing: We are able to prepare custom Neomycin Sulfate suitable for your unique specifications for use in cell culture, or as ancillary material in upstream biopharma manufacturing. Additionally, we offer testing for endotoxin content, arsenic content, cell line toxicity, spectral analysis, and more. For more information, please contact us.
| Mechanism of Action | Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein. |
| Spectrum | Neomycin is a broad-spectrum antibiotic, however, it is mostly used against Gram-negative bacteria. |
| Impurity Profile | Impurity A| 2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-streptamine (neamine or neomycin A-LP)|||| Impurity B| 3-N-acetyl-2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-streptamine (3-acetylneamine)|||| Impurity C| 4-O-(2-amino-2-deoxy-d-glucopyranosyl)-2-deoxy-d-streptamine (paromamine or neomycin D)|||| Impurity D| 2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-5-O-[3-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-ribofuranosyl]-d-streptamine (neomycin C)|||| Impurity E| 4-O-(2-amino-2-deoxy-d-glucopyranosyl)-2-deoxy-5-O-[3-O-(2,6-diamino-2,6-dideoxy-l-idopyranosyl)-d-ribofuranosyl]-d-streptamine (paromomycin I or neomycin E)|||| Impurity F| 4-O-(2-amino-2-deoxy-d-glucopyranosyl)-2-deoxy-5-O-[3-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-ribofuranosyl]-d-streptamine (paromomycin II or neomycin F)|||| Impurity G| 3-N-acetyl-2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-5-O-[3-O-(2,6-diamino-2,6-dideoxy-l-idopyranosyl)-d-ribofuranosyl]-d-streptamine (neomycin B-LP)|||| |
| Microbiology Applications |
Neomycin can be used for gene selection, via exploiting the resistance gene (NPT II) (Aragão, 2009). Neomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative effective ranges include:
For a representative list of Neomycin MIC values, click here. Neomycin Sulfate can be used for food testing in TSN agar to select for Clostridium perfringins and inhibit growth of Enterobacteria and Clostridum bifermentans. |
| Plant Biology Applications | Neomycin is commonly used in negative selections for plants which have been successfully been transformed with a plasmid conferring resistance via Agrobacterium mediated transformation. |
| Eukaryotic Cell Culture Applications | Neomycin is routinely used to select for cells containing resistance plasmids such as pcDNA3 in common cell lines including AtT-20. For additional information please visit our cell-culture database. |
| Molecular Formula |
C23H46N6O13 |
| References |
Aragão FJL and Brasileiro ACM (2002) Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol. 14(1):1-10 Dai et al (2001) Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Mol. Breeding 7: 25–33:2001 Davis BD (1987) Mechanism of bactericidal action of aminoglycosides. Microbiol. Rev. 51(3):341-50 Robertson JH (1971) Antimicrobial activity of Neomycin C against Staphylococcus epidermidis. App. Micro. 22(6):1164-1165 Tsuji K and Robertson JH (1969) Comparative study of responses to Neomycins B and C by microbiological and gas-liquid chromatographic assay methods. App. Microbiol. 18(3):396-398 Yuan L and Wei H (2006) Rapid analysis of native Neomycin components on a portable capillary electrophoresis system with potential gradient detection. Analytic. Bioanalyt. Chem. 385(8):1575-1579 |
| MIC | Bacillus cereus (CCM 99)| 7.29 － ?| 862| Diplococcus pneumoniae| 200 － 400| 1413| Enterococcus| ≤0.125 － 32| 1036| Enterococcus faecalis (ATCC 29212)| 6.25 － ?| 862| Escherichia coli| ? － ?| 1200| Escherichia coli| 0.25 － >512| 436| Escherichia coli| 0.5 － >64| 1| Escherichia coli (animal health isolate)| 1 － >64| 1| Escherichia coli (ATCC 25922)| 1.6 － ?| 737| Escherichia coli (ATCC 25922)| 4 － ?| 1379| Escherichia coli (ATCC 25922)| 128 － ?| 1472| Escherichia coli (ATCC 39628)| 5.73 － ?| 862| Haemophilus influenzae| 1.6 － 6.3| 1413| Haemophilus parasuis (Spain)| 4 － 64| 768| Haemophilus parasuis (UK)| 4 － 64| 768| Klebsiella pneumoniae| 1 － 256| 1379| Klebsiella pneumoniae| 1 － 256| 1379| Klebsiella pneumoniae (ATCC 33018)| 64 － ?| 1472| Klebsiella pneumoniae (ATCC 700630)| 8 － ?| 1379| Kocuria rhizophila (ATCC 9341)| 3.37 － ?| 862| Lactobacillus acidophilus (P/N 601379 + oxgall-stressed)| 62.5 － ?| 740| Lactobacillus acidophilus (P/N 601379 + pH 2.0)| 250 － ?| 740| Lactobacillus acidophilus (P/N 601379)| 62.5 － ?| 740| Lactobacillus acidophilus (R052 + oxgall-stressed)| 125 － ?| 740| Lactobacillus acidophilus (R052 + pH 2.0)| 125 － ?| 740| Lactobacillus acidophilus (R052 + pH 4.0)| 125 － ?| 740| Lactobacillus acidophilus (R052)| 62.5 － ?| 740| Lactobacillus bulgaricus (P/N 601383 + oxgall-stressed)| 125 － ?| 740| Lactobacillus bulgaricus (P/N 601383 + pH 2.0)| 500 － ?| 740| Lactobacillus bulgaricus (P/N 601383 + pH 4.0)| 125 － ?| 740| Lactobacillus bulgaricus (P/N 601383)| 62.5 － ?| 740| Lactobacillus casei (R0215 + oxgall-stressed)| 125 － ?| 740| Lactobacillus casei (R0215 + pH 2.0)| 250 － ?| 740| Lactobacillus casei (R0215 + pH 4.0)| 125 － ?| 740| Lactobacillus casei (R0215)| 250 － ?| 740| Lactobacillus casei (R0256 + oxgall-stressed)| 250 － ?| 740| Lactobacillus casei (R0256 + pH 2.0)| 250 － ?| 740| Lactobacillus casei (R0256 + pH 4.0)| 250 － ?| 740| Lactobacillus casei (R0256)| 250 － ?| 740| Lactobacillus lactis (R0187 + oxgall-stressed)| 125 － ?| 740| Lactobacillus lactis (R0187 + pH 2.0)| 250 － ?| 740| Lactobacillus lactis (R0187 + pH 4.0)| 125 － ?| 740| Lactobacillus lactis (R0187)| 250 － ?| 740| Lactobacillus paracasei (P/N 601385 + oxgall-stressed)| 62.5 － ?| 740| Lactobacillus paracasei (P/N 601385 + pH 4.0)| 62.5 － ?| 740| Lactobacillus paracasei (P/N 601385)| 62.5 － ?| 740| Lactobacillus plantarum (P/N 601387 + oxgall-stressed)| 250 － ?| 740| Lactobacillus plantarum (P/N 601387 + pH 2.0)| 250 － ?| 740| Lactobacillus plantarum (P/N 601387 + pH 4.0)| 250 － ?| 740| Lactobacillus plantarum (P/N 601387)| 250 － ?| 740| Lactobacillus plantarum (R0202 + oxgall-stressed)| 125 － ?| 740| Lactobacillus plantarum (R0202 + pH 2.0)| 250 － ?| 740| Lactobacillus plantarum (R0202 + pH 4.0)| 62.5 － ?| 740| Lactobacillus plantarum (R0202)| 250 － ?| 740| Lactobacillus plantarum (R1078 + oxgall-stressed)| 2500 － ?| 740| Lactobacillus plantarum (R1078 + pH 2.0)| 250 － ?| 740| Lactobacillus plantarum (R1078 + pH 4.0)| 125 － ?| 740| Lactobacillus plantarum (R1078)| 500 － ?| 740| Lactobacillus plantarum (R1096 + oxgall-stressed)| 125 － ?| 740| Lactobacillus plantarum (R1096 + pH 2.0)| 250 － ?| 740| Lactobacillus plantarum (R1096 + pH 4.0)| 250 － ?| 740| Lactobacillus plantarum (R1096)| 250 － ?| 740| Lactobacillus reuteri| 0.25 － 4| 1036| Lactobacillus reuteri| 100 － ?| 1424| Lactobacillus rhamnosus (R0011 + oxgall-stressed)| 250 － ?| 740| Lactobacillus rhamnosus (R0011 + pH 2.0)| 250 － ?| 740| Lactobacillus rhamnosus (R0011 + pH 4.0)| 125 － ?| 740| Lactobacillus rhamnosus (R0011)| 250 － ?| 740| Lactobacillus rhamnosus (R1039 + oxgall-stressed)| 500 － ?| 740| Lactobacillus rhamnosus (R1039 + pH 2.0)| 250 － ?| 740| Lactobacillus rhamnosus (R1039 + pH 4.0)| 125 － ?| 740| Lactobacillus rhamnosus (R1039)| 250 － ?| 740| Lactobacillus salivarius| 0.5 － 32| 1036| Lactococcus| ≤0.125 － 32| 1036| Leuconostoc| ≤0.125 － 32| 1036| Micrococcus (ML8)| 16 － ?| 745| Nocardia asteroides| 0.8 － >=800| 1404| Pediococcus| ≤0.125 － 32| 1036| Pseudomonas aeruginosa| 32 － ?| 665| Pseudomonas aeruginosa (NCTC 10662)| 32 － ?| 401| Staphylococcus (coagulase-negative + Uruguay)| ≤1 － 64| 1391| Staphylococcus aureus| ? － ?| 1200| Staphylococcus aureus| ≤1.7 － 129| 604| Staphylococcus aureus (ATCC 6538P)| 27.08 － ?| 862| Staphylococcus aureus (ATCC 6571)| 0.12 － ?| 401| Staphylococcus aureus (community-acquired + methicillin-resistant)| ≤1.7 － 129| 604| Staphylococcus aureus (methicillin-resistant)| ≤1.7 － 129| 604| Staphylococcus aureus (methicillin-susceptible)| ≤1.7 － 109| 604| Staphylococcus aureus (Uruguay)| ≤1 － 64| 1391| Staphylococcus spp. (coagulase negative)| ≤1.7 － 129| 604| Streptococci (β-hemolytic)| ≤1.7 － 27.3| 604| Streptococcus agalactiae| ≤1.7 － 13.7| 604| Streptococcus bovis| 0.5 － 8| 1036| Streptococcus infantarius| 0.5 － 8| 1036| Streptococcus pyogenes| ≤1.7 － ?| 604| Treponema hyodysenteriae| 50 － >100| 1427| Vibrio harveyi| 12.5 － ?| 1428| Weissella spp.| ≤0.125 － 2| 1036| |
