Sodium Dodecyl Sulfate (SDS), Dust-Free™ Ultrapure is an innovative pellet form of SDS designed to prevent aerosolized dust when preparing SDS solutions. Dust inhalation from standard grade SDS can lead to coughing, shortness of breath, and nasal passage irritation. Our R&D team has shown Dust-Free™ SDS dissolves 50% faster than traditional SDS.
SDS coats proteins and DNA giving them a negative charge allowing travel towards the anode during gel electrophoresis. It is an organic compound and anionic surfactant. Its hydrocarbon 'tail' coupled with the polar 'headgroup' yield amphiphilic characteristics that make it a useful detergent. The product is ≥99.5% pure.
We also offer:
- Sodium Dodecyl Sulfate, UltraPure (S057)
| Mechanism of Action | SDS works by denaturing proteins but does so without breaking peptide bonds. Once proteins are denatured, they carry the highly negative charge they acquired from the SDS, changing their shape and making them more suitable for gel electrophoresis. |
| Microbiology Applications | SDS can be used as a selective agent/supplement to inhibit the growth of non-coliform organisms. |
| Electrophoresis Applications |
SDS is used in several electrophoresis applications including SDS-PAGE gel construction, running buffers, and DNA and protein preparation and denaturing. It is used to lyse cells during RNA or DNA extraction. For SDS-PAGE applications, it disrupts non-covalent bonds in the proteins which results in denaturation. By binding to proteins at a ratio of 1 SDS moledule per 2 amino acid residues, the negatively charged SDS provides proteins with a net negative charge. Therefore, the mobility of the polypeptide chains can be attributed to their length which aids in analysis. |
| Solubility |
1% EtOH: Clear and colorless 10% Water: Clear and colorless |
