Clavulanate Acid Potassium with Cellulose (1:1) is a stable salt preparation of Clavulanic Acid, beta-lactam compound that functions as a natural β-lactamase inhibitor. It has weak, broad-spectrum activity but offers synergistic antibacterial activity with penicillins and cephalosporins for greater efficacy against β-lactam resistant strains. Cellulose serves as a microcrystalline,biocompatible, inert stabilizer and provides stability to the formulation as follows: Due to its porosity, cellulose can adsorb moisture and protect the hygroscopic Potassium Clavulanate from moisture and degradation. Clavulanic Acid was discovered in 1974 (Beecham) and its name is derived from strains of Streptomyces clavuligerus, which produces Clavulanic Acid. Clavulanate Acid Potassium with Cellulose (1:1) can be used for analysis, formulation development, and for optimizing processes for β-lactam antibiotics. Clavulanate is often used to enhance the antibacterial activity of amoxicillin and it is often used in combination.
We also offer:
| Mechanism of Action |
Clavulanate is a potent 'suicide inhibitor' of β-lactamases. It forms a covalent bond with a serine residue in the enzyme’s active site. Inhibition is time-dependent and irreversible. The compound binds to and inhibit PBPs (penicillin binding protein) thus inhibiting bacterial cell wall synthesis. Clavulanic acid potassium salt inhibits the amoxycillin-destroying action of many different types of β-lactamase sch as the staphylococcal enzyme, clinically important plasmid mediated enzymes of the TEM, SHV, OXA and PSE types, and the chromosomally-controlled enzymes made by Proteus mirabilis and Klebsiella pneumoniae. |
| Spectrum |
Gram-positive bacteria, Gram-negative bacteria. Clavulanic acid has negligible intrinsic antimicrobial activity. It has poor activity against S.aureus, Enterobacteriaceae, and P. aeruginosa. However, it can act synergistically with penicillins and cephalosporins to inhibit β-lactamase-producing S. aureus and Enterobacteriaceae. |
| Microbiology Applications |
Clavulanic Acid had poor activity against S.aureus, Enterobacteriaceae, and P. aeruginosa. However, it can act synergistically with penicillins and cephalosporins to inhibit β -lactamase-producing enteric bacteria to inhibit sensitive β-lactamase, thus allowing the companion β-lactam to kill the target bacteria. Clavulanic acid acts synergistically with penicillins and cephalosporins against β-lactamase-producing enteric bacteria to inhibit sensitive β-lactamases, thus allowing the companion β-lactam to kill the bacteria. Clavulanic Acid is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (broth microdilution) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include: Helicobacter pylori: <0.01 － 0.64 µg/ml For list of Clavulanic Acid MIC values, click here: https://antibiotics.toku-e.com/antimicrobial_1716.html Potassium Clavulanate is commonly combined with Amoxicillin (ie co-amoxiclav; trade name Augmentin); or Ticarcillin (co-ticarclav; trade name timentin). |
| Assay | 48.0-53.5% (on dried basis) (Clavulanic Acid Potassium) |
| Molecular Formula | C8H8KNO5 ∙ (C6H10O5)n |
| Impurities | n-Butanol: ≤5000ppm Impurity E: ≤1.0% Impurity G: ≤1.0% Any other impurity: ≤0.2% Total Impurities: ≤2.0% |
| References |
Balcazar-Ochoa LG et al (2024) Clavulanic Acid and its potential therapeutic effects on the central nervous system. Arch. Med. Res. 55(1):102916 PMID 38039802 Brown AG et al (1984) Clavulanic acid and its derivatives. Structure elucidation of clavulanic acid and the preparation of dihydroclavulanic acid, isoclavulanic acid, esters and related oxidation products. J. Chem. Soc. Perkin Trans. I 635 PMID Brown AGJ (1976) Naturally occurring beta-lactamase inhibitors with antibacterial activity. J. Antibiot. 29:668 PMID 950324 Bush K and Bradford PA (2016) β-Lactams and β-lactamase inhibitors: An overview. Cold Spring Harb Perspect. Med. 6(8):a025247. doi: 10.1101 PMID 27329032 Cole M (1982) Biochemistry and action of Clavulanic acid. Scott Med J. 1982;27 Spec No.:S10-6. PMID 6762660 Neu HC and Fu KP (1978) Clavulanic Acid, a novel inhibitor of β-Lactamases. Antimicrob. Agents and Chemother. 14 (5):650-655 PMID 310279 Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in Gram-negative bacilli. Am J Med 103:51 PMID 9236486 Reading C and Cole M (1977) Clavulanic Acid: a Beta-Lactamase-Inhibiting Beta-Lactam from Streptomyces clavuligerus. Antimicrob. Agents Chemother. 11(5):852-857 Saudagar PS, Survase SA and Singhal RS (2008) Clavulanic acid: A review. Bitechnol. Adv. 26(4):335-351 PMID 18450406 Wise R, Andrews JM, and Bedford KA (1978) In vitro study of Clavulanic Acid in combination with penicillin, amoxycillin, and carbenicillin. Antimicrob. Agents. Chemother. 13 (3):389-393 PMID 122520 |
