PCR Mycoplasma Detection Kit II is an improved version of the PCR Mycoplasma Detection Kit. Improvements include a modified Mycoplasma Primer Mix which helps to detect additional Mycoplasma and Acholeplasma strains when paired with the optimal cycling protocol. It also includes an internal PCR positive control to distinguish between a true negative result and a false-negative result.
The PCR Mycoplasma Detection Kit II quickly and reliably identifies Mycoplasma contamination in cell cultures. Mycoplasma infection can induce cellular changes, like chromosome aberrations, morphological changes and altered cell growth. The kit has a high degree of sensitivity for over 300 Mycoplasma strains while minimizing the risk of false positives. It is important to screen for Mycoplasma in suspected cell cultures to deter more widespread contamination. The MasterMix contains gel loading dye for convenient gel loading. The Kit contains reagents for 100 reactions.
The PCR Mycoplasma Detection Kit II contains the following:
1.25 ml 2x PCR Taq Master Mix II
100 µl Mycoplasma PCR Primer Mix II
250 µl Positive PCR Control
1.00 ml Nuclease-Free H20
Please see Product Data Sheet for kit protocol.
We also offer the following kits for Mycoplasma elimination:
| Eukaryotic Cell Culture Applications | Mycoplasma DNA in the cell culture supernatant is amplified via PCR and visualized using gel electrophoresis. In addition to the short detection process, the ease of handling and high sensitivity makes this product a convenient tool for routine examination of cell cultures and media. Please consult the Product Data Sheet for the protocol. Protocol note: in order to visualize the Mycoplasma DNA under UV light, you will need to add Ethidium Bromide. |
| References |
McGarrity GJ, Vanaman V and Sarama J (1984) Cytogenetic effects of mycoplasmal infection of cell cultures: A review. In Vitro 20:1–18 PMID 6199287 Romorini L et al (2013) Effect of antibiotics against Mycoplasma sp. on human embryonic stem cells undifferentiated status, pluripotency, cell viability and growth. PLOS ONE 8(7): e70267 Uphoff CC, Drexler HG (2002) Comparative PCR analysis for detection of Mycoplasma infections in continuous cell lines. In Vitro Cell.Dev.Biol.-Animal 38:79–85 |
