SKU: P038  / 
    CAS Number: 80469-10-9

    Polymyxin B1-I Sulfate, EvoPure®


    Polymyxin B1-I Sulfate, EvoPure® (syn: Isoleucine (Ile)-B1) is one of the individual polypeptide components in Polymyxin B Sulfate. Polymyxin B1-I contains, among other amino acids, an L-isoleucine residue in place of the L-leucine reside found in the other polymyxin fractions. The unique fatty acid group found in B1-I is 6-methyloctanoic acid (6-MOA), the same group found in Polymyxin B1. Results from in vitro studies have shown marginal differences in MIC data when comparing the fractions.

    We also offer:

    • Polymyxin B Sulfate, USP (P007)
    • Polymyxin B1 Sulfate, EvoPure® (P037)
    • Polymyxin B2 Sulfate, EvoPure® (P039)
    • Polymyxin B3 Sulfate, EvoPure® (P040)
    • Polymyxin B6 Sulfate, EvoPure® (P054)
    • Polymyxin E1 Sulfate, EvoPure® (P055)
    • Polymyxin E2 Sulfate, EvoPure® (P056)

    EvoPure® products are purified single antibiotic fractions, most are >99% pure. Highly pure EvoPure® Polymyxin products can be used to analyze the specifc effects of individual Polymyxin B fractions.

    Mechanism of Action Polymyxin B targets and alters permeability lipopolysaccharide (LPS) of Gram negative bacteria leading to lysing of the cell. Polymyxin B only needs to interact with LPS, it is not required to enter the cell.
    Spectrum Polymyxin B sulfate targets the outer membrane of Gram-negative bacteria especially Pseudomonas aeruginosa.

    Polymyxin B3 was the most active polymyxin fraction according to research done by Kassamali et al, and there was a synergistic effect using Polymyxin B3 and B1-I (Kassamali et al, year)
    Microbiology Applications Polymyxin B Sulfate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.  Representative MIC values include:
    • Pseudomonas aeruginosa 0.25 µg/mL – 1 µg/mL
    • For a complete list of polymyxin B sulfate MIC values, click here.
    Plant Biology Applications Polymyxin B sulfate was successfully tested to counteract phytopathogenic Gram-negative bacterial growth including different strains of Pseudomonas viridiflava and Erwinia carotovora. Polymixin B sulfate was shown to reduce bacterial growth of different strains of Pseudomonas viridiflava at low concentrations, (0.08 µg/ml) and Erwinia carotovora growth at slightly higher concentrations (0.25 µg/ml) (Selim et al. 2005).
    Molecular Formula C56H98N16O13  xH2O4S (lot specific)

    Kassamali, et al. used Polymyxin B1polymyxin B2polymyxin B3, and polymyxin B1-I (TOKU-E) to test for synergistic and antagonistic effects against various Gram-negative organisms.
    Read more here: "Microbiological Assessment of Polymyxin B Components Tested Alone and In Combination" 

    Lim et al. used Polymyxin B1, polymyxin B2polymyxin B3, and polymyxin B1-I (TOKU-E) to study the stability of each compound in saline, dextrose, and saline/dextrose infusion solutions in: "Physicochemical stability study of Polymyxin B in various infusion solutions for administration to critically Ill patients."  

    Cao G et al (2009)  Development and validation of a reversed-phase high-performance liquid chromatography assay for Polymyxin B in human plasma. J. Antimicrob. Chemother.  62(5):1009-1014 PMID 18765414

    Mueller MJ, Brodschelm W, Spannagl E and Zenk MH (1993)  Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid.  Proc. Natl. Acad. Sci. USA 90(16):7490-7494  PMID 11607420

    Newton BA (1956)  The properties and mode of action of the Polymyxins. Bacter. Rev.  20(1):14-27  PMID 13303920

    Orwa JA et al (2001)  Isolation and structural characterization of Polymyxin B components. J Chromatogr A 912(2):369-373  PMID 11330807

    Selim S, Negrel J, Govaerts C, Gianinazzi S and Tuinen van D (2005)  Isolation and partial characterization of antagonistic peptides produced by Paenibacillus sp. strain B2 isolated from the sorghum Mycorrhizosphere. Appl. Environ. Microbiol. 71(11):6501–6507  PMID 16269674

    Tam VH, Cao H, Ledesma, KR, Hu M (2011)  In vitro potency of various Polymyxin B components.55(9):4490-491  PMID 21709096

    Zavascki AP, Goldani LA, Li J and Nation RL (2007)  Polymyxin B for the treatment of multidrug-resistant pathogens: A critical review. J. Antimicrob. Chemother.  60(6):1206-1215  PMID 17878146

    MIC P. aeruginosa (ATCC 27853)| 4 |1666| A. baumannii (ATCC BAA 747)|1|1666| K. pneumoniae ATCC 13883|2|1666| P. aeruginosa (9019)|1|1666| A. baumannii (1261)|1|1666| K. pneumoniae (VM9)|1|1666|