StemGuardTM is an antibiotic reagent for laboratory grown stem cells to protect them from contamination from bacteria and Mycoplasma. It is a sterile-filtered, cell culture tested reagent with a concentration of 50 mg/ml. This proprietary blend is non-toxic to stem cells and can be added directly to the culture medium. It is used to protect pluripotent stem cells such as embryonic stem cells and induced pluripotent stem cells. Toxicity testing with human-induced pluripotent stem cells (hiPSC ) and Chinese Hamster Ovary (CHO) cells revealed StemGuard was non-toxic to these cell types in vitro. StemGuard is an alternative to Penicillin-Streptomycin (Pen-Strep).
StemGuard is used at a working concentration of 100 μg/ml.
We also offer:
- Penicillin-Streptomycin Solution (100X) (P030)
| Mechanism of Action | StemGuard inhibits protein synthesis in bacteria and Mycoplasmas. |
| Spectrum | StemGuard is broad-spectrum antibiotic formulation with activity against Gram-positive bacteria, Gram-negative bacteria and Mycoplasma. |
| Eukaryotic Cell Culture Applications |
The influence of Pen-Strep, Amphotericin B, a complex of Amphotericin B with copper (II) ions, and various combinations on the proliferation and differentiation of adipose-derived stem cells was evaluated in vitro. Results indicate that the influence of antibiotics depends on the duration of exposure and on the combination of compounds. The addition of Streptomycin changes the gene expression profile and may affect the differentiation process (Skubis et al, 2017). Stem cell contamination can lead to loss of cell viability, changes in cellular behavior and gene expression, and interference with downstream applications such as transplantation or gene editing. An important application for stem cell technology is tissue engineering involving the generation of cells and tissues for cell-based therapies. Scientists can differentiate iPSCs to create “organoids” or tissue chips for studying diseased cells and testing different compounds. |
| References |
Mahmood A and Ali S (2017) Microbial and viral contamination of animal and stem cell cultures: common contaminants, detection and elimination . J. Stem Cell Res. Ther. 2(5):149-155 Skubis A et al (2017) Impact of antibiotics on the proliferation and differentiation of human adipose-derived mesenchymal stem cells. Int J Mol Sci. 18(12):2522. PMID 29186789 van der Sanden B, Dhobb M, Berger F, Wion D (2010) Optimizing stem cell culture. J Cell Biochem.;111(4):801-7 PMID 20803548 |
