SKU: B013  / 
    CAS Number: 71048-99-2

    Bialaphos sodium - bar gene selective agent

    $148.54 - $645.39

    Bialaphos sodium is a freely soluble herbicide synthesized by Streptomyces hygroscopicus and Streptomyces viridochromeogenes.  It is commonly used for gene selection in cereals.

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    Mechanism of ActionBialaphos acts as a prodrug by separating into individual subunits of alanylalanine and phosphinothricin. Phosphinothricin is toxic to cells by triggering the accumulation of ammonia, and inhibiting photosynthesis and glutamine synthesis.
    Microbiology ApplicationsBIalaphos Sodium from TOKU-E was used to study its effects on oligopeptide transporters in a Sinorhizobium meliloti hfq mutant. (Sobrero et al, 2011)
    Plant Biology ApplicationsBialaphos Sodium is used in transformation of cereals including wheat, rice, maize, barley, sorghum, oat and rye. The bar gene is incorporated in the plant genome along with the gene of interest. When Bialaphos is applied, only plants which are successfully transformed survive (Aragão, 2002).
    Molecular FormulaC11H21N3NaO6P
    ReferencesAragão FJL and Brasileiro ACM (2001) Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol. 14(1):1-10

    Dedicova B, Bermudez C, Prias M, Zuniga E, Brondani C (2015) High-throughput transformation pipeline for a Brazilian japonica rice with bar gene selection. Protoplasma 252(4):1071-83 PMID 25488347

    Imai, S et al (2012) Conversion of Bialaphos to other oligopeptides containing phosphinothricin by Streptomyces Hygroscopicus. J. Antibiotics 44 (9):1006-1012

    Sobrero P et al (2012) Quantitative proteomic analysis of the Hfq-regulon in Sinorhizobium meliloti. PLoS One 7(10):e48494. PMID 23119037