Cefotetan Disodium is a broad-spectrum cephamycin antibiotic. Cefotetan is often grouped together with second-generation cephalosporins and has a similar antibacterial spectrum, but with additional coverage against anaerobic bacteria. It is also used as a media supplement in Oxford Listeria Selective Supplement. Cefotetan is also used in antimicrobial susceptibility testing.
| Mechanism of Action | Like β-lactams, cephamycins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to β-lactam antibiotics is commonly due to cells containing plasmid encoded β-lactamases, however, many cephamycins are unaffected by this mechanism. |
| Spectrum | Cefotetan is a broad-spectrum antibiotic targeting a wide variety of gram-positive and gram-negative bacteria. Like many cephamycins, cefotetan is very effective against anaerobic bacteria such as Bacteroides and Clostridium species. |
| Microbiology Applications | Cefotetan is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against aerobic and anaerobic gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:
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| Molecular Formula | C17H15N7Na2O8S4 |
| References |
Georgopapadakou NH (1992) Mechanisms of action of cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli. Antimicrob. Agents Chemother. 37(3):559-565 |
| MIC | Agrobacterium radiobacter| 0.25 － 8| 1448| Alcaligenes faecalis| 0.5 － >64| 1448| Bacteroides distasonis| 32 － >128| 1473| Bacteroides distasonis| <=0.06 － 256| 1468| Bacteroides fragilis| <=0.06 － 256| 1468| Bacteroides fragilis| 4 － >128| 1473| Bacteroides fragilis (1995)| 2 － 512| 297| Bacteroides fragilis (1996)| 1 － 512| 297| Bacteroides fragilis (group)| 0.06 － >=256| 1480| Bacteroides fragilis (group)| <=0.06 － 256| 1468| Bacteroides fragilis (group)| 4 － 128| 1399| Bacteroides fragilis (group)| 2 － >128| 1473| Bacteroides ovatus| 64 － >128| 1473| Bacteroides thetaiotaomicron| 2 － 256| 1468| Bacteroides thetaiotaomicron| 8 － >128| 1473| Bacteroides uniformis| 2 － 128| 1473| Bacteroides uniformis| <=0.06 － 256| 1468| Bacteroides vulgatus| 4 － 128| 1468| Bifidobacterium spp.| 0.5 － >128| 1473| Bordetella bronchiseptica| 8 － >64| 1448| Brevundimonas vesicularis| 8 － >64| 1448| Burkholderia cepacia| 8 － >64| 1448| Chryseobacterium indologenes| 4 － >64| 1448| Chryseobacterium meningosepticum| 64 － >64| 1448| Clostridium bifermentans| 0.25 － 4| 1473| Clostridium cadaveris| 0.25 － 4| 1473| Clostridium difficile| 8 － 32| 1473| Clostridium sordellii| 0.25 － 4| 1473| Clostridium spp.| 0.25 － 4| 1473| Clostridium tertium| 0.25 － 4| 1473| Comamonas acidovorans| 4 － ?| 1448| Escherichia coli (NCTC 10418)| 0.06 － ?| 401| Eubacterium lentum| 0.5 － >128| 1473| Finegoldia magna| 0.25 － 4| 1452| Fusobacteria| 0.06 － 8| 1473| Fusobacterium necrophorum| 0.06 － 0.125| 1473| Fusobacterium nucleatum| 0.125 － 0.5| 1473| Fusobacterium varium| 0.5 － 4| 1473| Lactobacillus spp.| 0.5 － >128| 1473| Ochrobactrum anthropi| 16 － >64| 1448| Oligella ureolytica| 0.13 － >64| 1448| Parvimonas micra| 0.125 － 2| 1452| Peptoniphilus gorbachii| 0.5 － 1.5| 1452| Peptoniphilus harei| 0.38 － 8| 1452| Peptoniphilus ivorii| 0.125 － 1| 1452| Peptoniphilus lacrimalis| 0.016 － 0.38| 1452| Peptoniphilus octavius| 0.5 － ?| 1452| Peptostreptococcus anaerobius| 0.25 － 2| 1473| Peptostreptococcus anaerobius| 0.5 － 24| 1452| Peptostreptococcus asaccharolyticus| 0.25 － 2| 1473| Peptostreptococcus magnus| 0.25 － 2| 1473| Peptostreptococcus tetradius| 0.25 － 2| 1473| Porphyromonas| 0.125 － 64| 1473| Porphyromonas asaccharolytica| 0.125 － 64| 1473| Prevotella buccae| 0.125 － 64| 1473| Prevotella corporis| 0.25 － 8| 1473| Prevotella disiens| 0.5 － 64| 1473| Prevotella intermedia| 0.5 － 8| 1473| Prevotella melaninogenica| 0.5 － 16| 1473| Prevotella oralis| 0.125 － 64| 1473| Prevotella oris| 0.125 － 64| 1473| Propionibacterium acnes | 0.25 - 1| 1473| Propionibacterium spp.| 0.25 － 1| 1473| Pseudomonas aeruginosa| >64 － ?| 1448| Pseudomonas aeruginosa (ATCC 27853)| >128 － ?| 401| Pseudomonas aeruginosa (NCTC 10662)| >128 － ?| 401| Pseudomonas oryzihabitans| 0.5 － >64| 1448| Pseudomonas stutzeri| <=0.06 － >64| 1448| Ralstonia pickettii| 8 － >64| 1448| Ruminococcus gnavus| 32 － ?| 1452| Sphingomonas spp.| 64 － >64| 1448| Staphylococcus aureus (ATCC 6571)| 4 － ?| 401| Stenotrophomonas maltophilia| 8 － >64| 1448| Weeksella virosa| 0.5 － 8| 1448| |
